- Clone
- QA20B07 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Macrosialin
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_QA20B07_A647_CD68_Antibody_061423 A_QA20B07_A647_CD68_Antibody_061423](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA20B07_A647_CD68_Antibody_061423.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes and permeabilized with 0.5% Triton X-100. Then, the tissue was stained with 10 µg/mL of anti-human CD68 recombinant (clone QA20B07) Alexa Fluor® 647 (red) and anti-human CD8a (clone C8/144B) Alexa Fluor® 594 (green) at 4°C overnight. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. -
Human paraffin-embedded prostate tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes and permeabilized with 0.5% Triton X-100. Then, the tissue was stained with 10 µg/mL of anti-human CD68 recombinant (clone QA20B07) Alexa Fluor® 647 (red) at 4°C overnight. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
381203 | 25 µg | $250 | ||||
381204 | 100 µg | $370 |
CD68, also known as macrosialin, is a 110 kD glycoprotein which belongs to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 – 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
Antigen Details
- Structure
- Sialomucin family, 110 kD
- Distribution
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Monocytes/macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, endothelial cells
- Molecular Family
- CD Molecules
- Antigen References
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- Holness CL and Simmons DL. 1993. Blood. 81:1607-13.
- Gottfried E, et al. 2008. Scand J Immunol. 67:453-63.
- Hameed A, et al. 1994. Hum Pathol. 25:872-6.
- Gene ID
- 968 View all products for this Gene ID
- UniProt
- View information about CD68 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD68 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD68 Recombinant Antibody | QA20B07 | IHC-P |
Alexa Fluor® 647 anti-human CD68 Recombinant Antibody | QA20B07 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD68 Recombinant Antibody
Human paraffin-embedded tonsil tissue slices were prepared w... -
Alexa Fluor® 647 anti-human CD68 Recombinant Antibody
Human paraffin-embedded tonsil tissue slices were prepared w... Human paraffin-embedded prostate tissue slices were prepared...
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