- Clone
- 28-8-6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class I
- Isotype
- Mouse (C3H) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 mouse splenocytes stained with 28-8-6 Alexa Fluor® 647
Cat # | Size | Price | Quantity Check Availability | Save | ||
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114612 | 100 µg | $229 |
The 28-8-6 antibody reacts with the H-2Kb and H-2Db MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Db haplotype. H-2Kb/H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2Dd MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C3H.SW mouse splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.
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Application References
(PubMed link indicates BioLegend citation) -
- Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt)
- Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC)
- Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed.
- Shao H, et al. 2005. J. Immunol. 175:1851. (FC)
- Product Citations
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- RRID
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AB_492931 (BioLegend Cat. No. 114612)
Antigen Details
- Structure
- MHC class I
- Distribution
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Nucleated cells of the H-2Kb/H-2Db haplotype
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
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1. Ozato K, et al. 1981. J. Immunol. 126:317.
2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447.
3. Evans GA, et al. 1992. Nature 300:755. - Gene ID
- 14964 View all products for this Gene ID 14972 View all products for this Gene ID
- UniProt
- View information about H-2Kb/H-2Db on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All H-2Kb/H-2Db Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-mouse H-2Kb/H-2Db | 28-8-6 | FC |
Purified anti-mouse H-2Kb/H-2Db | 28-8-6 | FC,IHC-F |
Biotin anti-mouse H-2Kb/H-2Db | 28-8-6 | FC |
PE anti-mouse H-2Kb/H-2Db | 28-8-6 | FC |
Alexa Fluor® 647 anti-mouse H-2Kb/H-2Db | 28-8-6 | FC |
PerCP/Cyanine5.5 anti-mouse H-2Kb/H-2Db | 28-8-6 | FC |
APC/Fire™ 750 anti-mouse H-2Kb/H-2Db | 28-8-6 | FC |
APC anti-mouse H-2Kb/H-2Db | 28-8-6 | FC |
PE/Cyanine7 anti-mouse H-2Kb/H-2Db | 28-8-6 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse H-2Kb/H-2Db
C57BL/6 mouse splenocytes were stained with H-2Kb... -
Purified anti-mouse H-2Kb/H-2Db
C57BL/6 mouse splenocytes were stained with purified H-2K -
Biotin anti-mouse H-2Kb/H-2Db
C57BL/6 mouse splenocytes were stained with biotinylated H-2... -
PE anti-mouse H-2Kb/H-2Db
C57BL/6 mouse splenocytes stained with 28-8-6 PE -
Alexa Fluor® 647 anti-mouse H-2Kb/H-2Db
C57BL/6 mouse splenocytes stained with 28-8-6 Alexa Fluor® 6... -
PerCP/Cyanine5.5 anti-mouse H-2Kb/H-2Db
C57BL/6 mouse splenocytes were stained with H-2Kb/H-2Db (clo... -
APC/Fire™ 750 anti-mouse H-2Kb/H-2Db
C57BL/6 mouse splenocytes were stained with H-2Kb... -
APC anti-mouse H-2Kb/H-2Db
C57BL/6 mouse splenocytes were stained with H-2Kb... -
PE/Cyanine7 anti-mouse H-2Kb/H-2Db
C57BL/6 mouse splenocytes were stained with H-2Kb...
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