- Clone
- DX9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NKAT3, NKB1, KIR-NKB1, p70
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood lymphocytes stained with DX9 Alexa Fluor® 700
Cat # | Size | Price | Quantity Check Availability | Save | ||
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312711 | 25 µg | $147 | ||||
312712 | 100 µg | $300 |
CD158e1, also known as NKB1, is a 70 kD member of the immunoglobulin superfamily that is expressed on a subset of natural killer cells and T cells at varying levels among individuals. NKB1 is a type I membrane protein containing two immunoglobulin C2-type domains. The interaction of NKB1 with specific HLA-B antigens on a target cell (the HLA-Bw4 allele, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human NK cell clone VL186-1.6
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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The DX9 antibody reacts with the KIR (killer cell inhibitory receptor) designated NKB1 or KIR3DL1. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and restoring the NK cell cytotoxicity4,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312710).
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Application References
(PubMed link indicates BioLegend citation) -
- Litwin V, et al. 1994. J. Exp. Med. 180:537. (IP)
- Gumperz J, et al. 1996. J. Exp. Med. 183:1817.
- Gardiner CM, et al. 2001. J. Immunol. 166:2992.
- Bakker ABH, et al. 1998. J. Immunol. 160:5239.
- Goodier M, et al. 2000. J. Immunol. 165:139.
- Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006. (FC)
- Yawata M, et al. 2002. Immunogenetics 54:543.
- Valiante NM, et al. 1997. Immunity 7:739.
- Pascal V, et al. 2007. J. Immunol. 179:1625. (FC) PubMed
- Lichterfeld M, et al. 2008. J. Exp. Med. 204:2813. (FC) PubMed
- Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
- Purdy AK, et al. 2014. J Immunol. 193:4675. PubMed
- Product Citations
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- RRID
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AB_2234188 (BioLegend Cat. No. 312711)
AB_2130824 (BioLegend Cat. No. 312712)
Antigen Details
- Structure
- Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, 70 kD type I membrane protein
- Distribution
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Subset of NK and T cells
- Function
- Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-B antigens on target cell
- Ligand/Receptor
- HLA-B antigens such as the HLA-Bw4 allele
- Cell Type
- NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Colonna M, et al. 1995. Science 268:405.
2. D'Andrea A, et al. 1995. J. Immunol.. 155:2306.
3. Uhrburg M, et al. 1997. Immunity 7:753.
4. Gumperz JE, et al. 1996. J. Exp. Med. 183:1817.
5. Wagtmann N, et al. 1995. Immunity 3:801. - Gene ID
- 3811 View all products for this Gene ID
- UniProt
- View information about CD158e1 on UniProt.org
Related Pages & Pathways
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD158e1 (KIR3DL1, NKB1)
Human peripheral blood lymphocytes stained with DX9 FITC -
PE anti-human CD158e1 (KIR3DL1, NKB1)
Human peripheral blood lymphocytes stained with DX9 PE and C... -
Alexa Fluor® 700 anti-human CD158e1 (KIR3DL1, NKB1)
Human peripheral blood lymphocytes stained with DX9 Alexa Fl... -
Brilliant Violet 421™ anti-human CD158e1 (KIR3DL1, NKB1)
Human peripheral blood lymphocytes were stained with CD56 AP... -
PerCP/Cyanine5.5 anti-human CD158e1 (KIR3DL1, NKB1)
Human peripheral blood lymphocytes were stained with CD56 AP... -
PE/Cyanine7 anti-human CD158e1 (KIR3DL1, NKB1)
Human peripheral blood lymphocytes were stained with CD56 FI... -
APC anti-human CD158e1 (KIR3DL1, NKB1)
Human peripheral blood lymphocytes were stained with CD56 PE... -
APC/Fire™ 750 anti-human CD158e1 (KIR3DL1, NKB1)
Human peripheral blood lymphocytes were stained with CD56 PE... -
TotalSeq™-A0599 anti-human CD158e1 (KIR3DL1, NKB1)
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TotalSeq™-C0599 anti-human CD158e1 (KIR3DL1, NKB1)
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TotalSeq™-B0599 anti-human CD158e1 (KIR3DL1, NKB1)
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Brilliant Violet 650™ anti-human CD158e1 (KIR3DL1, NKB1)
Human peripheral blood cells were surface stained with anti-... -
Brilliant Violet 711™ anti-human CD158e1 (KIR3DL1, NKB1)
Human peripheral blood cells were surface stained with anti-... -
Spark Red™ 718 anti-human CD158e1 (KIR3DL1, NKB1) (Flexi-Fluor™)
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Brilliant Violet 605™ anti-human CD158e1 (KIR3DL1, NKB1)
Human peripheral blood lymphocytes were stained with anti-hu...
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