- Clone
- P2B1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Platelet endothelial cell adhesion molecule, PECA1, GPIIA', endoCAM, CD31 antigen, CD31/EndoCAM, adhesion molecule, PECAM-1, CD31/EndoCAM
- Previously
-
Covance Catalog# MMS-484R
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![P2B1_PECAM_Ascites_IHC_031615 P2B1_PECAM_Ascites_IHC_031615](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/P2B1_PECAM_Ascites_IHC_031615.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Staining of PECAM using Clone P2B1 on frozen human kidney. -
Immunofluorescence of HUVEC cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) CD31 antibody (Clone P2B1). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG (Cat. No. 405319) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/15, and (B-D) is 1/30. Concentrations for (A, B) is 2 µg/ml, (C) is 1 µg/ml and (D) is 0.5 µg/ml.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
910003 | 100 µL | $253 |
PECAM (platelet endothelial cell adhesion molecule) is expressed on activated T-cells and endothelial cells and is also known as CD31. The protein is involved in transmigration of leukocytes across endothelium during inflammation. P2B1 activates the binding of human T-lymphocytes to endothelial cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- The P2B1 monoclonal antibody was generated against IL-1β activated human umbilical vein endothelial cells (HUVECs).
- Preparation
- Ascites
- Concentration
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
- Application
-
IHC-F - Quality tested
ICC, WB, IP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a dilution range of 1:500 - 1:1000 is suggested. For immunocytochemistry, a range of 1:250 - 1:500 is recommended. For Western blotting, the suggested use of this reagent is 1:1000 dilution. For immunoprecipitation, the suggested use of this reagent is 1:1000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting, immunocytochemistry (ICC), immunoprecipitation (IP) and immunohistochemistry (IHC).
*Predicted MW = 83 kD
Positive Control: human kidney -
Application References
(PubMed link indicates BioLegend citation) -
- Brooks PC, et al. 1995. J Clin Invest. 96:1815. (IF)
- Product Citations
-
- RRID
-
AB_2565394 (BioLegend Cat. No. 910003)
Antigen Details
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroinflammation, Neuroscience, Signal Transduction, Synaptic Biology, Transcription Factors
- Molecular Family
- Adhesion Molecules, CD Molecules
- Gene ID
- 5175 View all products for this Gene ID
- UniProt
- View information about CD31 on UniProt.org
Related FAQs
Other Formats
View All CD31 (PECAM-1) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-CD31 (PECAM-1) | P2B1 | IHC-F,ICC,WB,IP |
Purified anti-CD31 (PECAM-1) | P2B1 | IHC-F,ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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