- Clone
- 8RB13 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
- Previously
-
NeoClone Catalog# S0004
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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673801 | 1 mL | $323 |
RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.
Product DetailsProduct Details
- Verified Reactivity
- E. coli
- Reported Reactivity
- Other species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant E. coli RNA polymerase β subunit.
- Formulation
- 50% anti- E. coli RNA Polymerase antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
- Storage & Handling
- Store at 4°C until use. Use at room temperature.
- Application
-
IP - Quality tested
- Recommended Usage
-
Resin binding capacity is approximately 0.15 mg/mL. To elute, use TE, 0.7M ammonium sulfate, 40% propylene glycol.
- Application Notes
-
Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.
Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3. -
Application References
(PubMed link indicates BioLegend citation) -
- Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
- Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
- Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)
Antigen Details
- Structure
- 1252 amino acids with a predicted molecular weight of approximately 150 kD.
- Distribution
-
Bacteria.
- Function
- Transcriptional regulator in bacteria.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. - Gene ID
- 948488 View all products for this Gene ID
- UniProt
- View information about RNA Polymerase beta on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All RNA Polymerase β Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-E. coli RNA Polymerase ImmunoAffinity Resin | 8RB13 | IP |
Anti-E. coli RNA Polymerase β | 8RB13 | WB,ELISA,IP |
Purified anti-E. coli RNA Polymerase β | 8RB13 | WB,ELISA,IP |
Direct-Blot™ HRP anti-E. coli RNA Polymerase β | 8RB13 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-E. coli RNA Polymerase ImmunoAffinity Resin
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Anti-E. coli RNA Polymerase β
Total cell lysates (15 µg total protein) from E. Coli Total cell lysates (15 µg protein) from E. Coli str... -
Purified anti-E. coli RNA Polymerase β
Total lysates (15 µg protein) from E. coli BL21 cells were r... -
Direct-Blot™ HRP anti-E. coli RNA Polymerase β
Total cell lysates (15 µg protein) from HeLa and E.coli BL21...
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