- Clone
- 38F3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RRNA 2'-O-methyltransferase fibrillarin, 34 kD nucleolar scleroderma antigen, RNA, U3 small nucleolar interacting protein 1
- Previously
-
Covance Catalog# MMS-581S
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
905001 | 200 µL | $313 |
Nop1p was originally identified as a nucleolar protein of bakers yeast, S. cerevisiae (accession P15646). The Nop1p protein is 327 amino acids in size (34.5kD), is essential for yeast viability, and is localized in the nucleoli. It is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein apparently found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This means that suitably cross-reactive antibodies to Nop1p/fibrillarin, like MCA-38F3, can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archea tested to date.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, Drosophila
- Reported Reactivity
- Other species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- 38F3 was raised against yeast nuclear preparations.
- Formulation
- Contains 0.01M sodium azide.
- Preparation
- Tissue Culture Supernatant
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
ICC, KO/KD-WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 0.5 µg per mL (dilution 1:500 - 1:1000). For immunocytochemistry, a dilution range of 1:500 (mammalian), and 1:1000 - 1:5000 (yeast) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Reactivity to Drosophila was only verified with the purified format.
This antibody is useful for immunoblotting (WB), immunofluorescence (ICC) and immunocytochemistry.
*Predicted MW = 34 kD - Additional Product Notes
-
For Western blotting, the suggested use of this reagent is 1:500-1:2000 dilution in Drosophila and 1:500-1:1000 dilution in other species. For immunocytochemistry, a dilution range of 1:500 (mammalian), 1:100 - 1:500 (Drosophila) and 1:1000 - 1:5000 (yeast) is recommended.
-
Application References
(PubMed link indicates BioLegend citation) -
- Newton K, et al. 2003. Mol. Cell Biol. 23:8519.
- Ochs RL, et al. 1985. Biol. Cell. 54:123.
- Lessard F, et al. 2012. Nucelic Acids Res. 40:5357. (IF) PubMed
- Scruggs BS. et al. 2012. Mol Cell Biol. 32:4092. (WB) PubMed
- Lessard F, et al. 2010. Moll Cell. 38:539. (IF, WB)
- Denissov S, et al. 2011. EMBO Rep. 12:231. (IF) PubMed
- Product Citations
-
- RRID
-
AB_2565045 (BioLegend Cat. No. 905001)
Antigen Details
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID
- 2091 View all products for this Gene ID
- UniProt
- View information about Fibrillarin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Fibrillarin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-Fibrillarin | 38F3 | WB,ICC,KO/KD-WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Anti-Fibrillarin
Western blot analysis of cell lysates from A549, HEL (human)... Total lysates (15 µg protein) from 293T and 293T/Fibrillarin... Human HEK293 cell stained with mouse monoclonal antibody to ... Western blot of purified anti-Fibrillarin antibody (clone 38... ICC staining of anti-Fibrillarin antibody (clone 38F3) on Dr...
Follow Us