- R1-2 (See other available formats)
- Other Names
- α4 integrin, VLA-4 α chain, integrin α4, ITGA4
- Rat IgG2b, κ
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- Product Citations
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CD49d is a 150 kD glycoprotein, also known as α4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin.Product Details
- Antibody Type
- Host Species
- AKR/Cum mouse spontaneous T lymphoma line TK1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
The R1-2 antibody has been shown to partially block CD49d mediated interactions and, in combination with the 9C10 (MFR4.B) antibody, completely blocks VCAM-1 binding to VLA-4. Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and T cell costimulation1-4, immunoprecipitation1, and immunohistochemistry of frozen sections5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103629 and 103630).
(PubMed link indicates BioLegend citation)
- Holzmann B, et al. 1989. Cell 56:37. (IP, Block)
- Ferguson TA, et al. 1993. J. Immunol. 150:1172. (Block)
- Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
- Kilshaw PJ, et al. 1991. Eur. J. Immunol. 21:2591. (Block)
- Jaspers M, et al. 1995. Differentiation 59:79. (IHC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
AB_2565776 (BioLegend Cat. No. 103621)
AB_2565777 (BioLegend Cat. No. 103622)
- Integrin family, associates with integrin β1 or β7, 150 kD
T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells
- T cell costimulation, cell-cell and cell-matrix adhesion
- VCAM-1, MAdCAM-1, fibronectin
- Cell Type
- B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994 J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.
- Gene ID
- 16401 View all products for this Gene ID
- View information about CD49d on UniProt.org
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