APC anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Other Names
FcRI
Isotype
Mouse IgG1, κ
Ave. Rating
6 reviews
Product Citations
publications
X54-57.1_1_APC_1_052912
C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD64 (clone X54-5/7.1) APC (top) or mouse IgG1, κ APC isotype control (bottom). Data shown was gated on the myeloid population.
  • X54-57.1_1_APC_1_052912
    C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD64 (clone X54-5/7.1) APC (top) or mouse IgG1, κ APC isotype control (bottom). Data shown was gated on the myeloid population.
  • X54-57.1_1_APC_2_052912
See APC spectral data
Cat # Size Price Quantity Avail. Save
139305 25 µg $90
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139306 100 µg $225
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical data sheet

Product Details

Reactivity
Mouse CD64 a and b alloantigens.
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
Product Citations
  1. Zhou Q, et al. 2014. J Immunol. 193:496. PubMed
  2. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  3. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  4. Terhorst D, et al. 2015. J Immunol. 195: 4953 - 4961. PubMed
  5. Qayum A, et al. 2016. J Immunol. 196: 4457 - 4467. PubMed
  6. Meliopoulos V, et al. 2016. PLoS Pathog. 12: 1005804. PubMed
  7. Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed
  8. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  9. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  10. Zwick M, et al. 2019. Front Immunol. 10:222. PubMed
  11. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  12. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  13. Wang KC, et al. 2018. Immunohorizons. 2:407. PubMed
  14. Zhang S, et al. 2019. Mol Med Rep. 19:5377. PubMed
  15. Qi X, et al. 2019. Nat Commun. 10:2141. PubMed
  16. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  17. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  18. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
  19. Steuerman Y et al. 2018. Cell systems. 6(6):679-691 . PubMed
  20. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  21. de Tymowski C et al. 2019. Cell reports. 27(3):762-775 . PubMed
  22. Yu‐Han Chang et al. 2017. Immunity. 47(5):943-958 . PubMed
  23. Holly Bachus et al. 2018. Immunity. 50(1):225-240 . PubMed
  24. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  25. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
  26. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  27. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
  28. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  29. Huang WC, et al. 2019. Int J Hyperthermia. 36:1255. PubMed
RRID
AB_11219205 (BioLegend Cat. No. 139305)
AB_11219391 (BioLegend Cat. No. 139306)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/11/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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