- Clone
- miap301 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Integrin-associated protein, IAP, neurophilin, gp42, Rh-associated protein
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Miap301_APCCy7_CD47_Antibody_112216 Miap301_APCCy7_CD47_Antibody_112216](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Miap301_APCCy7_CD47_Antibody_112216.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD47 (clone miap301) APC/Cyanine7 (filled histogram) or rat IgG2a, κ APC/Cyanine7 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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127525 | 25 µg | $171 | ||||
127526 | 100 µg | $422 |
CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking2 and immunohistochemical staining of frozen sections3. This clone is not cross-reactive with human CD47.
- Additional Product Notes
- BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Wang H, et al. 2007. P. Natl. Acad. Sci. USA 104:13744.
- Jiang P, et al. 1999 J. Biol. Chem. 274:559. (IHC-F, Block)
- Product Citations
-
- RRID
-
AB_2632861 (BioLegend Cat. No. 127525)
AB_2632862 (BioLegend Cat. No. 127526)
Antigen Details
- Structure
- 50 kD plasma membrane protein of the immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids, and a short cytoplasmic tail of 30 amino acids.
- Distribution
-
Broad tissue distribution, including haemopoietic cells, epithelial and endothelial cells, fibroblasts, erythrocytes and other tissue cells.
- Function
- Adhesion molecules, immune regulation.
- Interaction
- Associates with β3 integrins.
- Ligand/Receptor
- SIRPα (CD172α, Signal regulatory protein alpha), thrombospondin.
- Bioactivity
- Upon interaction with SIRPα, controls innate immune effector function, such as host cell phagocytosis.
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Brown E, et al. 1990. J. Cell. Biol. 111:2785.
2. Mawby WJ, et al. 1994. Biochem. J. 304:525.
3. Gao AG, et al. 1996. J. Biol. Chem. 271:21.
4. Oldenborg PA, et al. 2000. Science 288:2051.
5. van Beek EM, et al. 2005. J. Immunol. 175:7781. - Gene ID
- 16423 View all products for this Gene ID
- UniProt
- View information about CD47 on UniProt.org
Related FAQs
Other Formats
View All CD47 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD47 | miap301 | FC,IHC-F |
FITC anti-mouse CD47 | miap301 | FC |
Biotin anti-mouse CD47 | miap301 | FC |
PE anti-mouse CD47 | miap301 | FC |
Alexa Fluor® 647 anti-mouse CD47 | miap301 | FC,IHC-F |
APC anti-mouse CD47 | miap301 | FC |
PerCP/Cyanine5.5 anti-mouse CD47 | miap301 | FC |
Ultra-LEAF™ Purified anti-mouse CD47 | miap301 | FC,Block |
PE/Cyanine7 anti-mouse CD47 | miap301 | FC |
PE/Dazzle™ 594 anti-mouse CD47 | miap301 | FC |
APC/Cyanine7 anti-mouse CD47 | miap301 | FC |
Brilliant Violet 421™ anti-mouse CD47 | miap301 | FC |
Alexa Fluor® 488 anti-mouse CD47 | miap301 | FC |
Alexa Fluor® 594 anti-mouse CD47 | miap301 | IHC-F |
TotalSeq™-A1031 anti-mouse CD47 | miap301 | PG |
TotalSeq™-B1031 anti-mouse CD47 | miap301 | PG |
TotalSeq™-C1031 anti-mouse CD47 | miap301 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD47
C57BL/6 splenocytes stained with miap301 purified, followed ... C57BL/6 mouse frozen liver section was fixed with 4% parafor... -
FITC anti-mouse CD47
Balb/c mouse splenocytes stained with Miap301 FITC -
Biotin anti-mouse CD47
BALB/c splenocytes stained with Miap301 biotin, followed by ... -
PE anti-mouse CD47
Balb/c mouse splenocytes stained with Miap301 PE -
Alexa Fluor® 647 anti-mouse CD47
BALB/c mouse splenocytes stained with Miap301 Alexa Fluor® 6... BALB/c mouse frozen spleen section was fixed with 4% parafor... -
APC anti-mouse CD47
C57BL/6 mouse splenocytes were stained with CD47 (clone miap... -
PerCP/Cyanine5.5 anti-mouse CD47
C57BL/6 mouse splenocytes were stained with CD47 (clone miap... -
Ultra-LEAF™ Purified anti-mouse CD47
-
PE/Cyanine7 anti-mouse CD47
C57BL/6 mouse splenocytes were stained with CD47 (clone miap... -
PE/Dazzle™ 594 anti-mouse CD47
C57BL/6 mouse splenocytes were stained with CD47 (clone miap... -
APC/Cyanine7 anti-mouse CD47
C57BL/6 mouse splenocytes were stained with CD47 (clone miap... -
Brilliant Violet 421™ anti-mouse CD47
C57BL/6 mouse splenocytes were stained with CD47 (clone miap... -
Alexa Fluor® 488 anti-mouse CD47
C57BL/6 spelnocytes were stained with anti-mouse CD47 (clone... -
Alexa Fluor® 594 anti-mouse CD47
BALB/c mouse frozen spleen section was fixed with 4% parafor... -
TotalSeq™-A1031 anti-mouse CD47
-
TotalSeq™-B1031 anti-mouse CD47
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TotalSeq™-C1031 anti-mouse CD47
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