Biotin anti-human CCL17 (TARC) Antibody

Pricing & Availability
Clone
Poly5230 (See other available formats)
Other Names
Thymus and activation-regulated chemokine (TARC), Small-inducible cytokine 17 (SCYA17), ABCD-2
Isotype
Goat Polyclonal Ig
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523003 50 µg $355
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Description

CCL17 was initially isolated from phytohemagglutinin-stimulated PBMCs. CCL17 is constitutively expressed in the thymus and, under activation, in several cell types. CCL17-mediated recruitment of Th2 cells and CLA+ CD4+ T cells plays a key role in allergic diseases like atopic dermatitis, allergic asthma, allergic rhinitis, and allergic contact dermatitis. In addition, CCL17 has been detected in idiopathic pulmonary fibrosis. Also, CCL17 and CCL22 secreted by dendritic cells seem to mediate the recruitment of regulatory T cells to sites of inflammation in patients with chronic hepatitis. CCL17 is upregulated by several combinations of cytokines: TNF-α, IL-4, and IFN-γ in bronchial epithelial cells; TGF-β1, TNF-α, and IFN-γ in human keratinocyte cell lines; and histamine and prostaglandin E2P in immature dendritic cells.    

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human CCL17
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-0.5 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of CCL17 (TARC) recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated Poly5230 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with the purified Poly5230 antibody (Cat. No. 523002) as the capture antibody and recombinant human CCL17 (TARC) (Cat. No. 573809) as the standard.

Note: For testing human CCL17 (TARC) in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 441107 & 441108) are specially developed and recommended.

RRID
AB_2562795 (BioLegend Cat. No. 523003)

Antigen Details

Structure
Chemokine.
Distribution
Upon activation, CCL17 is expressed by PBMC, monocytes, dendritic cells, bronchial epithelial cells, smooth muscle cells, keratinocytes, and fibroblasts.
Function
CCL17 chemoattracts T cells, Th2 cells, and CLA+ CD4+ T cells and plays major role in allergic diseases. CCL17 can be upregulated by IL-4 in T cells and by a combination of cytokines in different cells.
Interaction
T cells, Th2 cells, CLA+ CD4 T cells, T (reg) cells, NK cells, NKT cells, and macrophages.
Ligand/Receptor
CCR4.
Cell Type
Monocytes, Dendritic cells, Epithelial cells, Fibroblasts
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Imai T, et al. 1997. J. Biol. Chem. 272:15036.
2. McIlroy A, et al. 2006. Immunology 117:507.
3. Yogo Y, et al. 2009. Respir. Res. 10:80.
4. Stutte S, et al. 2010. Proc. Natl. Acad. Sci. USA 107:8736.
5. Oo YH, et al. 2010. J. Immunol. 184:2886.

Gene ID
6361 View all products for this Gene ID
UniProt
View information about CCL17 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 12/20/2013

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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