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Biotin anti-human IL-1β Antibody

Pricing & Availability
Clone
Poly5174 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly5174_Human_IL-1b_Antibody_ELISA_093014
  • Poly5174_Human_IL-1b_Antibody_ELISA_093014
Cat # Size Price Quantity Check Availability Save
517403 50 µg $375
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Description

IL-1 refers to two proteins, IL-1α and IL-1β, which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immunomodulator which mediates a wide range of immune and inflammatory responses, including the activation of B and T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human IL-1 beta
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-1β protein ranging from 1000 to 15.6 pg/ml are recommended for each ELISA plate when paired with H1b-27 (Cat. No. 511601). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated Poly5174 is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H1b-27 antibody (Cat. No. 511601) as the capture antibody and recombinant human IL-1β (Cat. No. 579409) as the standard.

Note: For testing human IL-1β in serum or plasma, BioLegend's LEGEND MAX™ Kits (Cat. No. 437007 & 437008) are specially developed and recommended.

RRID
AB_2561336 (BioLegend Cat. No. 517403)

Antigen Details

Structure
Cytokine, 17.3 kD.
Distribution

Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes.

Interaction
B cells, T cells, monocytes.
Ligand/Receptor
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b).
Cell Type
Astrocytes, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Langerhans cells, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

Gene ID
3553 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 02/01/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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