Biotin anti-MFF Antibody

Pricing & Availability
Clone
N382/14 (See other available formats)
Other Names
Mitochondrial Fission Factor, C2orf33, Chromosome 2 Open Reading Frame 33, EMPF2, GL004
Isotype
Mouse IgG1, κ
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Product Citations
publications
N382-14_Biotin_MFF_Antibody_1_120419.png
ICC staining of Biotin anti-MFF antibody (clone N328/14) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% bovine serum albumin. The cells were then stained with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) for 1 hour at room temperature. The cells were counterstained with Flash Phalloidin™ Green 488 (Cat. No. 424201) and DAPI to visualize actin filaments and nuclei, respectively. The image was captured with a 60X objective. Scale bar: 20 µm
  • N382-14_Biotin_MFF_Antibody_1_120419.png
    ICC staining of Biotin anti-MFF antibody (clone N328/14) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% bovine serum albumin. The cells were then stained with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) for 1 hour at room temperature. The cells were counterstained with Flash Phalloidin™ Green 488 (Cat. No. 424201) and DAPI to visualize actin filaments and nuclei, respectively. The image was captured with a 60X objective. Scale bar: 20 µm
  • N382-14_Biotin_MFF_Antibody_2_120419.png
    ICC staining of Biotin anti-MFF antibody (clone N328/14) on A549 cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% bovine serum albumin. The cells were then stained with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) for 1 hour at room temperature. The cells were counterstained with Flash Phalloidin™ Green 488 (Cat. No. 424201) and DAPI to visualize actin filaments and nuclei, respectively. The image was captured with a 60X objective. Scale bar: 20 µm.
Cat # Size Price Quantity Avail. Save
857505 25 µg $90
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857506 100 µg $160
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Description

Mitochondrial fission factor (MFF) is a mitochondrion outer membrane protein that is part of the mitochondrial fission complex where it acts a receptor for the dynamin-related protein 1 (Drp1). The MFF-Drp1 complex is responsible for the promotion of mitochondrial fission. MFF has also been shown to regulate peroxisome morphology.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein including amino acids 1-173 and 272-322 of human MFF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820141 (BioLegend Cat. No. 857505)
AB_2820142 (BioLegend Cat. No. 857506)

Antigen Details

Structure
MFF is a 342 amino acid protein with a molecular mass of ~38 kD.
Distribution

Tissue Distribution: Highly expressed in heart, kidney, liver, brain, muscle, and stomach.
Cellular Distribution: Mitochondria, peroxisome, nucleus, and cytosol.

Function
MFF plays a role in mitochondrial and peroxisomal fission by facilitating the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface.
Interaction
MFF interacts with the dynamin-related protein 1 (Drp1). MFF has also been shown to interact with DDX28 and ILF3.
Biology Area
Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience
Molecular Family
Organelle Markers
Antigen References
  1. Moon Yong Cha, et al. 2018. PLoS Curr. 26;10.
  2. Kornfeld OS, et al. 2018. Sci Rep. 19;8(1):14034.
  3. Otera H, et al. 2010. J Cell Biol. 13;191(6):1141-58.
  4. Liu R, et al. 2015. Mol Biol Cell. 1;26(24):4466-77.
Gene ID
56947 View all products for this Gene ID
UniProt
View information about MMF on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 12/04/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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