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Biotin anti-mouse CD140b Antibody

Pricing & Availability
Clone
APB5 (See other available formats)
Regulatory Status
RUO
Other Names
PDGF receptor-β, PDGFR-β, platelet-derived growth factor receptor beta
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
APB5_Biotin_030510
Mouse fibroblast NIH/3T3 cells stained with biotinylated APB5, followed by Sav-PE
  • APB5_Biotin_030510
    Mouse fibroblast NIH/3T3 cells stained with biotinylated APB5, followed by Sav-PE
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136009 25 µg $59
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136010 100 µg $165
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Description

Platelet-derived growth factor receptor-β (PDGFR-β), CD140b, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs. PDGFRβ is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140b is expressed on embryonic tissues and mesenchymal-derived cells adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PDGFR-β-hIgG1 recombinant fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for the relevant formats) applications include: Western blotting and blocking function2. The LEAF™ purified antibody is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Sano H, et al. 2001. Circulation 103:2955.
  2. Sano H, et al. 2002. Am. J. Pathol. 161:135. (Block)
Product Citations
  1. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  2. Ferreirinha P, et al. 2022. Development. 149: . PubMed
  3. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  4. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  5. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  6. Seong J, et al. 2018. Development. 145:14. PubMed
RRID
AB_2162325 (BioLegend Cat. No. 136009)
AB_2236916 (BioLegend Cat. No. 136010)

Antigen Details

Structure
Beta chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution

Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.

Function
Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand/Receptor
PDGFs
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Soriano P, et al. 1994. Genes Dev. 8:1888
2. Takakura N, et al. 1996. J Invest Dermatol. 107:770
3. Yarden Y, et al. 1986. Nature 323:226

Gene ID
18596 View all products for this Gene ID
UniProt
View information about CD140b on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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