Biotin anti-mouse CD30 Antibody

Pricing & Availability
Clone
mCD30.1 (See other available formats)
Other Names
Ber-H2, Ki-1, TNFRSF8
Isotype
Armenian Hamster IgG
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Product Citations
publications
mcd30.1
Con A-stimulated (3 days)splenocytes stained with purified mCD30.1, then detected with biotinylated anti-Armenian hamster IgG followed by Sav-PE
  • mcd30.1
    Con A-stimulated (3 days)splenocytes stained with purified mCD30.1, then detected with biotinylated anti-Armenian hamster IgG followed by Sav-PE
Cat # Size Price Quantity Avail. Save
102303 50 µg $65
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Description

CD30 is a 105 kD type I transmembrane glycoprotein, also known as Ber-H2 or Ki-1. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on activated T, B, and NK cells, and monocytes. CD28, in conjunction with several cytokines, regulates the expression of CD30 on activated T cells. It has been reported that cytokine production and Th1 versus Th2 development is heavily influenced by CD30-CD30 ligand (CD153) interactions.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse CD30-mouse IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro induction of IL-5 production1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 102305/102306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 102304) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102308).

Application References

(PubMed link indicates BioLegend citation)
  1. Bowen MA, et al. 1996. J. Immunol. 156:442.
  2. Punt JA, et al. 1997. J. Exp. Med. 186:1911.
  3. Ye Z, et al. 2010. Mol Immunol. 47:2411. PubMed
Product Citations
  1. Ye Z, et al. 2010. Mol Immunol. 47:2411. PubMed
RRID
AB_2240925 (BioLegend Cat. No. 102303)

Antigen Details

Structure
TNFR superfamily, 105-120 kD
Distribution
Activated T cells, B cells and NK cells, monocytes
Function
Costimulation, regulates negative selection of thymocytes
Ligand/Receptor
CD153 (CD30L)
Cell Type
T cells, B cells, NK cells, Monocytes, Embryonic Stem Cells, Tregs
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Falini B, et al. 1995. Blood 85:1.
3. Gedrich RW, et al. 1996. J. Biol. Chem. 271:12852.

Gene ID
21941 View all products for this Gene ID
UniProt
View information about CD30 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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