- Clone
- A16097B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Microtubule associated protein tau
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A16097B_Biotin_Tau359-373_Antibody_1_012219 A16097B_Biotin_Tau359-373_Antibody_1_012219](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A16097B_Biotin_Tau359-373_Antibody_1_012219.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of Biotin anti-Tau, 359-373 antibody (clone A16097B). Lane 1: Molecular weight marker; Lane 2: 20 µg of normal human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system. -
IHC staining of Biotin anti-Tau, 359-373 antibody (clone A16097B) on formalin-fixed paraffin-embedded normal human (left panel) and Alzheimer’s disease (right panel) brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissues were incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). The slides were counterstained with hematoxylin, according to the protocol provided. Scale Bar: 40 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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851405 | 25 µg | $136 | ||||
851406 | 100 µg | $300 |
Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyperphosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of human 4R Tau protein (K18) encompassing the microtubule binding domains.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
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WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This antibody cross-reacts with 3R and 4R human Tau isoforms, and recognizes endogenous human, mouse, and rat Tau in brain lysates by WB.
- RRID
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AB_2801197 (BioLegend Cat. No. 851405)
AB_2801198 (BioLegend Cat. No. 851406)
Antigen Details
- Structure
- Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
- Distribution
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Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol. - Function
- Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
- Interaction
- Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Tau
- Antigen References
- Gene ID
- 4137 View all products for this Gene ID
- UniProt
- View information about Tau 359-373 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All Tau, 359-373 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Tau, 359-373 | A16097B | WB,Direct ELISA,IHC-P |
Biotin anti-Tau, 359-373 | A16097B | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Tau, 359-373
Western blots of anti-Tau, 359-373 antibody (clone A16097B) ... IHC staining of anti-Tau, 359-373 antibody (clone A161097B) ... Direct ELISA of purified anti-Tau, 359-373 antibody (clone A... -
Biotin anti-Tau, 359-373
Western blot of Biotin anti-Tau, 359-373 antibody (clone A16... IHC staining of Biotin anti-Tau, 359-373 antibody (clone A16...
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