Biotinylated Recombinant Human CD38 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
CD38, T10, CD38 antigen (p45), CD38 antigen, CD38 molecule, cluster of differentiation 38, Cyclic ADP-ribose hydrolase 1, CyclicADP-ribose Hydrolase, NAD(+) nucleosidase
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Recom_Human_CD38_1_CF
When purified anti-human CD38 antibody is immobilized at 1 µg/mL, recombinant human CD38 binds in a dose-dependent manner. HRP avidin (Cat. No. 405103) was used to detect the binding.
  • Recom_Human_CD38_1_CF
    When purified anti-human CD38 antibody is immobilized at 1 µg/mL, recombinant human CD38 binds in a dose-dependent manner. HRP avidin (Cat. No. 405103) was used to detect the binding.
  • Recom_Human_CD38_2_CF
    Stability testing for Recombinant Human CD38. Recombinant human CD38 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were evaluated in a binding assay with immobilized purified anti-human CD38 antibody at 1 µg/mL and different concentration of human CD38 that binds in a dose-dependent manner. The EC50 range for this effect is 0.7 – 4.2 ng/mL.
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568104 25 µg $375
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568106 100 µg $735
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Description

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscle, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells, natural killer (NK) lymphocytes, myeloblasts, and erythroblasts. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38. ADP-ribosyl cyclase is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose.

Product Details
Technical data sheet

Product Details

Source
Biotinylated recombinant human CD38, amino acid Val 43 - Ile 300 (Accession No.NP_001766.2), with a C-terminal Avi-6His tag, was expressed in CHO cells. Human CD38-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 294 amino acid recombinant protein has a predicted molecular mass of approximately 33.5 kD. The DTT-reduced and non-reduced protein migrates at approximately 35 – 50 kD by SDS-PAGE. The predicted N-terminal amino acid is Val.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH7.2.
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Immobilized purified anti-human CD38 antibody at 1 µg/mL binds recombinant human CD38 in a dose-dependent manner. HRP avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 0.7 – 4.2 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
Distribution

T cells, B cells, NK-cells, myeloid, plasma, and dendritic cells

Function
Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
Interaction
NK cells, Neutrophils, Macrophages
Ligand/Receptor
CD31, hyaluronic acid
Bioactivity
Measured by its ability to bind CD38 antibody
Cell Type
B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Gunawan M, et al. 2017. Sci Rep. 7:16642.
  2. Martin JC, et al. 2020. Cell. 178:1493.
  3. Del Alcazar D, et al 2019. Cell Rep. 28:3047-3060.e7.
  4. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262-1277.
  5. Cole C, et al. 2018. Nucleic Acids Res. 46:e62.
  6. Lavin Y, et al. 2017. Cell. 169:750-765.
Gene ID
952 View all products for this Gene ID
UniProt
View information about CD38 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/10/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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