- Clone
- TB28-2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin light chain kappa, Ig kappa
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![TB28-2_BV421_Ig-light-chain-k_Antibody_070618 TB28-2_BV421_Ig-light-chain-k_Antibody_070618](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/TB28-2_BV421_Ig-light-chain-k_Antibody_070618.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Overnight cultured human peripheral blood lymphocytes were stained with CD3 APC/Cy7 and Ig light chain k (clone TB28-2) Brilliant Violet 421™ (left) or mouse IgG1, κ isotype control Brilliant Violet 421™ (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
392705 | 25 tests | $171 | ||||
392706 | 100 tests | $402 |
The TB28-2 antibody reacts with both soluble and membrane human immunoglobulin light chain kappa (κ). It does not react with human immunoglobulin light chain lambda (λ) or heavy chain.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IgG κ myeloma protein CFA.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
The TB28-2 target antigen is also present in serum. Cell surface staining may be optimized by removing serum from the sample prior to staining.
Additional reported applications (for relevant formats) include immunohistochemical staining on frozen tissue sections1. -
Application References
(PubMed link indicates BioLegend citation) -
- Moller P, et al. 1989. Am. J. Clin. Pathol. 1:18. (IHC-F)
- Tierens AM, et al. 2010. Haematologica. 95:1334. (FC)
- Product Citations
-
- RRID
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AB_2750128 (BioLegend Cat. No. 392705)
AB_2750129 (BioLegend Cat. No. 392706)
Antigen Details
- Structure
- Ig family
- Distribution
-
B-Cells
- Cell Type
- B cells
- Biology Area
- Immunology
- Antigen References
-
- Katzmann JA, et al. 2002. Clin Chem. 48: 1437
- Weinberg DS, et al. 1984. Blood. 63:1080.
- Johnson A, et al. 1985. Br. J. Cancer. 52: 159.
- Oka S, et al. 2012. J. Clin. Exp. Hematop. 52: 127.
- Gene ID
- 3514 View all products for this Gene ID
- UniProt
- View information about Ig light chain kappa on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All Ig light chain κ Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Ig light chain κ | TB28-2 | FC,IHC-F |
Brilliant Violet 421™ anti-human Ig light chain κ | TB28-2 | FC |
PE anti-human Ig light chain κ | TB28-2 | FC |
APC anti-human Ig light chain κ | TB28-2 | FC |
FITC anti-human Ig light chain κ | TB28-2 | FC |
PE/Cyanine7 anti-human Ig light chain κ | TB28-2 | FC |
Spark Violet™ 538 anti-human Ig light chain κ | TB28-2 | FC |
Brilliant Violet 605™ anti-human Ig light chain κ | TB28-2 | FC |
Pacific Blue™ anti-human Ig light chain κ | TB28-2 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
Brilliant Violet 421™ anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
PE anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
APC anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
FITC anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
PE/Cyanine7 anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
Spark Violet™ 538 anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
Brilliant Violet 605™ anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
Pacific Blue™ anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s...
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