- Clone
- 3A20.2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SAv
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![3A20-2_BV421_Streptavidin_Antibody_1_053024 3A20-2_BV421_Streptavidin_Antibody_1_053024](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3A20-2_BV421_Streptavidin_Antibody_1_053024.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 thymocytes were sequentially stained with biotinylated anti-mouse CD4 (clone RM4-5), Streptavidin APC, and anti-Streptavidin (clone 3A20.2) Brilliant Violet 421™ (filled histogram), or mouse IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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410505 | 50 µg | $320 |
Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including flow cytometry, molecular biology, and bionanotechnology.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Streptavidin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
The 3A20.2 purified antibody is useful as a third step reagent to amplify dim or low signals in many applications. This antibody does not cross-react with avidin.
Antigen Details
- Structure
- 52.8 kD tetrameric protein.
- Function
- Streptavidin binds to biotin with high affinity.
- Ligand/Receptor
- Biotin
- Antigen References
-
1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal Methods Chem. 58:1093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.
- Gene ID
- NA
- UniProt
- View information about Streptavidin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All Streptavidin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Streptavidin Antibody | 3A20.2 | FC |
PE anti-Streptavidin Antibody | 3A20.2 | FC |
Brilliant Violet 421™ anti-Streptavidin Antibody | 3A20.2 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Streptavidin Antibody
C57BL/6 thymocytes were sequentially stained with CD4 Biotin... -
PE anti-Streptavidin Antibody
C57BL/6 thymocytes were sequentially stained with CD4 Biotin... -
Brilliant Violet 421™ anti-Streptavidin Antibody
C57BL/6 thymocytes were sequentially stained with biotinylat...
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