- Clone
- UCHL1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV N31
- Other Names
- CD45RO
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![UCHL1_BV510_CD45RO_Antibody_FC_1_051915 UCHL1_BV510_CD45RO_Antibody_FC_1_051915](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/UCHL1_BV510_CD45RO_Antibody_FC_1_051915.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD45RA PE and CD45RO (clone UCHL1) Brilliant Violet 510™ (top) or mouse IgG2a, κ Brilliant Violet 510™ isotype control (bottom). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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304245 | 25 tests | $193 | ||||
304246 | 100 tests | $381 |
CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Cynomolgus, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- IL-2 dependent T cell line, CA1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
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The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5 and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.
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Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
- Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
- Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
- Yamada M, et al. 1996. Stroke 27:1155. (IHC)
- Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
- Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
- Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
- Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
- Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
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- RRID
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AB_2565800 (BioLegend Cat. No. 304245)
AB_2565801 (BioLegend Cat. No. 304246)
Antigen Details
- Structure
- Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
- Distribution
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Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- CD22
- Cell Type
- B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85. - Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RO on UniProt.org
Related Pages & Pathways
Pages
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 APC -
FITC anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 FITC -
PE anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 PE Human peripheral blood lymphocytes stained with UCHL1 PE and... -
PE/Cyanine5 anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 PE/Cya... -
Purified anti-human CD45RO
Human peripheral blood lymphocytes stained with purified UCH... SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
Alexa Fluor® 488 anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 Alexa ... Human paraffin-embedded tonsil tissue slices were prepared w... -
Pacific Blue™ anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 Pacifi... -
Alexa Fluor® 700 anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 Alexa ... -
Biotin anti-human CD45RO
Human peripheral blood lymphocytes stained with biotinylated... -
Brilliant Violet 421™ anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... Human paraffin-embedded tonsil tissue slices were prepared w... -
PerCP/Cyanine5.5 anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
Brilliant Violet 570™ anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
Brilliant Violet 605™ anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
APC/Cyanine7 anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
PE/Cyanine7 anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
Brilliant Violet 650™ anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
Brilliant Violet 711™ anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
Brilliant Violet 785™ anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
Alexa Fluor® 594 anti-human CD45RO
Human paraffin-embedded tonsil tissue slice was stained with... -
Purified anti-human CD45RO (Maxpar® Ready)
Human PBMCs stained with 153Eu-anti-CD45RA (HI100) and 164Dy... -
Brilliant Violet 510™ anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
PE/Dazzle™ 594 anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
APC/Fire™ 750 anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
PerCP anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RO ... -
APC anti-human CD45RO
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-A0087 anti-human CD45RO
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TotalSeq™-B0087 anti-human CD45RO
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TotalSeq™-C0087 anti-human CD45RO
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Brilliant Violet 750™ anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
PE/Fire™ 640 anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA ... -
TotalSeq™-D0087 anti-human CD45RO
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Spark Violet™ 423 anti-human CD45RO
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP APC anti-human CD45RO
Typical results from human peripheral blood lymphocytes stai... -
PE anti-human CD45RO
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-Bn1369 anti-human CD45RO
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GMP PE anti-human CD45RO
Typical results from human peripheral blood lymphocytes stai... -
Spark UV™ 387 anti-human CD45RO
Human peripheral blood cells were surface stained with anti-... -
APC/Fire™ 810 anti-human CD45RO
Human peripheral blood lymphocytes were stained with anti-hu...
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