- Clone
- HB15e (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HB15
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HB15e_BV711_CD83_Antibody_FC_012317 HB15e_BV711_CD83_Antibody_FC_012317](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HB15e_BV711_CD83_Antibody_FC_012317.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Monocyte-derived dendritic cells (induced with GM-CSF and IL-4 and TNF-α) stained with CD83 (clone HB15e) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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305333 | 25 tests | $215 | ||||
305334 | 100 tests | $435 |
CD83 is a 43 kD single chain type I glycoprotein also known as HB15. A member of the immunoglobulin superfamily, CD83 is expressed on a subset of dendritic cells, Langerhans cells, and weakly on activated lymphocytes. Although CD83 is thought to play a role in antigen presentation and/or lymphocyte activation, the precise function of this protein is unknown. CD83 is considered to be a useful marker for mature dendritic cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4.
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Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Zhou L, et al. 1995. J. Immunol. 154:3821.
- Cao W, et al. 2005. Biochem. J. 385:85.
- Lore K, et al. 2002. AIDS 16:683. (IHC)
- Cho H, et al. 2007. Physiol Genomics doi:10.1152/physiolgenomics.00051.2006
- RRID
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AB_2650748 (BioLegend Cat. No. 305333)
AB_2650749 (BioLegend Cat. No. 305334)
Antigen Details
- Structure
- Ig superfamily, single chain transmembrane glycoprotein, 43 kD
- Distribution
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Dendritic cells, Langerhan cells, activated B and T cells
- Cell Type
- B cells, Dendritic cells, Langerhans cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kozlow E, et al. 1993. Blood 81:454.
2. Zhou L, et al. 1992. J. Immunol. 149:735.
3. Zhou L, et al. 1995. Blood 86:3295. - Gene ID
- 9308 View all products for this Gene ID
- UniProt
- View information about CD83 on UniProt.org
Related FAQs
Other Formats
View All CD83 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
APC anti-human CD83 | HB15e | FC |
Biotin anti-human CD83 | HB15e | FC |
FITC anti-human CD83 | HB15e | FC |
PE anti-human CD83 | HB15e | FC |
PE/Cyanine5 anti-human CD83 | HB15e | FC |
Purified anti-human CD83 | HB15e | FC,IHC-F |
Alexa Fluor® 488 anti-human CD83 | HB15e | FC |
Alexa Fluor® 647 anti-human CD83 | HB15e | FC |
PerCP/Cyanine5.5 anti-human CD83 | HB15e | FC |
Brilliant Violet 421™ anti-human CD83 | HB15e | FC |
PE/Cyanine7 anti-human CD83 | HB15e | FC |
PE/Dazzle™ 594 anti-human CD83 | HB15e | FC |
APC/Cyanine7 anti-human CD83 | HB15e | FC |
Brilliant Violet 711™ anti-human CD83 | HB15e | FC |
APC/Fire™ 750 anti-human CD83 | HB15e | FC |
Brilliant Violet 605™ anti-human CD83 | HB15e | FC |
Brilliant Violet 785™ anti-human CD83 | HB15e | FC |
TotalSeq™-A0359 anti-human CD83 | HB15e | PG |
TotalSeq™-C0359 anti-human CD83 | HB15e | PG |
TotalSeq™-B0359 anti-human CD83 | HB15e | PG |
TotalSeq™-D0359 anti-human CD83 | HB15e | PG |
Brilliant Violet 510™ anti-human CD83 | HB15e | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC anti-human CD83
Monocytes-derived dendritic cells (induced with GM-CSF+IL-4+... -
Biotin anti-human CD83
Monocytes-derived dendritic cells (induced with GM-CSF+IL-4+... -
FITC anti-human CD83
Human monocyte-derived dendritic cells stained with HB15e FI... -
PE anti-human CD83
Monocyte-derived dendritic cells stained with HB15e PE -
PE/Cyanine5 anti-human CD83
Monocyte-derived dendritic cells (induced with GM-CSF+IL-4+T... -
Purified anti-human CD83
Monocyte-derived dendritic cells stained with HB15e PE -
Alexa Fluor® 488 anti-human CD83
Human monocytes-derived mature dendritic cells stained with ... -
Alexa Fluor® 647 anti-human CD83
Human monocytes-derived dendritic cells stained with HB15e A... -
PerCP/Cyanine5.5 anti-human CD83
Human monocyte-derived dendritic cells (induced with GM-CSF+... -
Brilliant Violet 421™ anti-human CD83
Human monocyte-derived dendritic cells (induced with GM-CSF+... -
PE/Cyanine7 anti-human CD83
Human monocyte-derived dendritic cells were stained with CD8... -
PE/Dazzle™ 594 anti-human CD83
Human monocyte-derived dendritic cells (induced with GM-CSF+... -
APC/Cyanine7 anti-human CD83
Human monocyte-derived dendritic cells (induced with GM-CSF ... -
Brilliant Violet 711™ anti-human CD83
Monocyte-derived dendritic cells (induced with GM-CSF and IL... -
APC/Fire™ 750 anti-human CD83
Human monocyte-derived dendritic cells (induced with GM-CSF ... -
Brilliant Violet 605™ anti-human CD83
Human monocyte-derived dendritic cells (induced with GM-CSF ... -
Brilliant Violet 785™ anti-human CD83
Human monocyte-derived mature dendritic cells (induced with ... -
TotalSeq™-A0359 anti-human CD83
-
TotalSeq™-C0359 anti-human CD83
-
TotalSeq™-B0359 anti-human CD83
-
TotalSeq™-D0359 anti-human CD83
-
Brilliant Violet 510™ anti-human CD83
Human monocyte-derived mature dendritic cells (induced with ...
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