- Clone
- 7/4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GKPIPNPLLGLDST epitope tag
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![7-4_DB-HRP_V5-tag_Antibody_031319 7-4_DB-HRP_V5-tag_Antibody_031319](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/7-4_DB-HRP_V5-tag_Antibody_031319.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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0.5 µL of Recombinant Posi-Tag Epitope Tag Protein (Cat No. 931301) and total cell lysates (15 µg total protein) from untransformed BL21 E. coli cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:4000 dilution of Direct-Blot™ HRP anti-V5-tag antibody, clone 7/4, overnight at 4°C. Proteins were visualized by chemiluminescence detection. Lane M: Molecular Weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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680604 | 25 µL | $101 | ||||
680603 | 100 µL | $340 |
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. - RRID
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AB_2734532 (BioLegend Cat. No. 680604)
AB_2572008 (BioLegend Cat. No. 680603)
Antigen Details
- Structure
- 14 amino acid peptide (GKPIPNPLLGLDST)
- Function
- The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
- Biology Area
- Cell Biology
- Antigen References
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1. Uhlen M, et al. 1990. Meth Enzymol. 185:129.
2. Hanke T, et al. 1995. J. Virol. Methods 53:149.
3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551.
4. Thomas SM, et al. 1988. Cell 54:891. - Gene ID
- NA
- UniProt
- View information about V5-tag on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All V5-tag Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-V5-tag | 7/4 | WB,IP |
Direct-Blot™ HRP anti-V5-tag | 7/4 | WB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-V5-tag
Different amounts of V5 tagged proteins (Genescript, M0101) ... 200ng V5 tagged protein was resuspended in PBS. Lane 1 was i... -
Direct-Blot™ HRP anti-V5-tag
0.5 µL of Recombinant Posi-Tag Epitope Tag Protein (Cat No. ...
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