FITC anti-rat CD49d Antibody

Pricing & Availability
MRα4-1 (See other available formats)
Other Names
Integrin α4 chain, α4 integrin, VLA-4 α chain, ITGA4
Mouse IgG2a, κ
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Product Citations
Lou rat blood lymphocytes stained with MRα4-1 FITC
  • MRα4-1
    Lou rat blood lymphocytes stained with MRα4-1 FITC
See FITC spectral data
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200103 50 µg $85
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CD49d is a 150 kD glycoprotein, also known as integrin α4 or VLA-4 α chain. It is expressed as a heterodimer with either of two β chains, β1 or β7. The α4β1 integrin (also known as VLA-4, CD49d/CD29) is expressed on peripheral T and B lymphocytes, thymocytes, and monocytes, while the α4β7 integrin (also known as LPAM-1) is expressed on peripheral lymphocytes. The α4β1 and α4β7 integrins mediate cell-cell and cell-matrix interactions by interacting with the ligands CD106 (VCAM-1), MAdCAM-1, and fibronectin. Soluble MRα4-1 antibody can partially inhibit in vitro binding of VCAM-1 and mast cells to fibronectin and inhibit mast cell degranulation. Immobilized MRα4-1 antibody can enhance mast cell degranulation in vitro.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
RBL-2H3 rat basophilic leukemia cell line
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunoprecipitation1, in vitro activation, in vitro inhibition1 of binding of VCAM-1 and mast cells to fibronectin , and in vivo inhibition of VLA-4 functions1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200106).

Application References

(PubMed link indicates BioLegend citation)
  1. Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP, Block)
Product Citations
  1. Greis C, et al. 2017. Crit Care. 10.1186/s13054-017-1654-9. PubMed
  2. Felce JH, et al. 2018. Sci Signal. 11:eaat0756. PubMed
  3. Jiang M, et al. 2020. Exp Ther Med. 1.989583333. PubMed
AB_2265193 (BioLegend Cat. No. 200103)

Antigen Details

Integrin α chain, 150 kD

α41- peripheral T cells and B cell lymphocytes, thymocytes, and monocytes; α47- peripheral lymphocytes

CD106 (VCAM-1), MAdCAM-1, fibronectin
Cell Type
B cells, Lymphocytes, Monocytes, T cells, Thymocytes
Biology Area
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Yasuda, M., et al. 1995. Int. Immunol. 7:251.
2. Springer, T.A. 1994. Cell 76:301.
3. Issekutz, T. B., et al. 1991. J. Immunol. 147:4178.
4. Issekutz, T. B., et al. 1991. J. Immunol. 147:109.
5. Yang, H., et al. 1995. Transplantation 60:71.
6. Palecanda, A., et al. 1997. J. Immunol. 158:2904.

Gene ID
311144 View all products for this Gene ID
View information about CD49d on

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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