FITC Avidin

Pricing & Availability
Other Names
Avidin-Fluorescein Isothiocyanate, Av-FITC
Ave. Rating
1 reviews
Description

Avidin binds to biotin with high affinity. Avidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.

Product Details
Cat # Size Price Quantity Avail. Save
405101 100 µg $30.00
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405102 500 µg $100.00
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Technical data sheet

Product Details

Formulation
10 mM HEPES, 0.5 M NaCl, pH 8.0, containing 0.08% sodium azide.
Preparation
Avidin is conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
This solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Avidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Avidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.

Application References

(PubMed link indicates BioLegend citation)

1. Jeon OH, et al. 2007. Throb res. 119:609. PubMed
2. Lin HY, et al. 2013. J. Virol. 87:9064. PubMed

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Go To Top Version: 2    Revision Date: 01/28/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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