LEGEND MAX™ Human APRIL/TNFSF13 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
A Proliferation Inducing Ligand (APRIL), TALL-2
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Product Citations
publications
APRIL_Human_LegMax_073012
  • APRIL_Human_LegMax_073012
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439307 1 Pre-coated Plate $462
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Description

A Proliferation-Inducing Ligand (APRIL), also known as TNFSF13, as its name suggests, stimulates cell proliferation. It is a member of the TNF superfamily of ligands, being most closely related to BAFF, with which it shares 30% amino acid sequence identity. APRIL is cleaved intracellularly by furin and is believed to exist mainly in a secreted, soluble form. As with most other TNF family members, APRIL exists as a functional homotrimer (total MW = 90 kD). It can bind to two cell surface receptors, BCMA and TACI, which it shares with BAFF to exert downstream T- and B-cell regulatory effects. It also possesses a heparin binding domain and has been demonstrated to bind to proteoglycans on the cell surface.

APRIL is most well known for its tumor proliferation effects. It is a potential biomarker, with serum levels elevated for certain cancers and expression levels elevated in cancer tissues. It is also a potential biomarker for certain autoimmune diseases. In fact, recombinant TACI is currently in clinical trials as a neutralization drug against APRIL and BAFF for the treatment of Systemic Lupus Erythematosus (SLE).

Besides forming homotrimers, APRIL can also form functional heterotrimers with BAFF. The stoichiometric relationship of the protomeric units is still unclear, however it appears that these heterotrimers are significant in that they are elevated in the serum of certain autoimmune patients.

APRIL also forms an endogenous chimeric protein with another related protein, TWEAK, effectively called TWE-PRIL. It is believed that TWE-PRIL is membrane bound and consists of the N-terminus of TWEAK with its transmembrane domain, and the C-terminus of APRIL with its receptor binding domain.


BioLegend's LEGEND MAX™ Human APRIL/TNFSF13 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip-well plate that is pre-coated with a monoclonal capture antibody. This kit is specifically designed for the accurate protein quantitation of human APRIL/TNFSF13 from cell culture supernatant, serum, plasma, and other biological fluids. It can detect APRIL-BAFF heterotrimers, in addition to APRIL homotrimers. It is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human APRIL/TNFSF13 Pre-coated 96-well Strip Microplate
  • Human APRIL/TNFSF13 Dectection Antibody
  • Human APRIL/TNFSF13 Standard
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human, Non-Human Primate
Application

ELISA

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. Szymula A, et al. 2023. Cell Rep. 112767:42. PubMed
  2. Madayag K, et al. 2022. Immun Inflamm Dis. 10:e729. PubMed
Sensitivity
0.038 ng/mL
Standard Range
0.16-10 ng/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Gene ID
8741 View all products for this Gene ID
UniProt
View information about APRIL on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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