- Other Names
- Tumor Necrosis Factor, Tumor Necrosis Factor Ligand Superfamily Member 2, Cachectin, TNF-Alpha, TNFSF2, TNF-A, TNFA, Tumor Necrosis Factor Ligand 1F, Tumor Necrosis Factor-Alpha, TNF Macrophage-Derived, TNF Monocyte-Derived, APC1 Protein, TNLG1F, DIF
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|740711||100 tests (270 µL)||$100|
The LEGENDplex™ Human Angiogenesis Panel 1 Mix and Match Capture Beads offer flexible customization within the panel (12-plex) using the following items listed below:
740597 LEGENDplex™ Human IL-6 Capture Bead A4, 13X
740701 LEGENDplex™ Human Angiopoietin-1 Capture Bead A5, 13X
740702 LEGENDplex™ Human Angiopoietin-2 Capture Bead A6, 13X
740703 LEGENDplex™ Human EGF Capture Bead A7, 13X
740704 LEGENDplex™ Human IL-34 Capture Bead A10, 13X
740705 LEGENDplex™ Human FGF-basic Capture Bead A10, 13X
740706 LEGENDplex™ Human CXCL8 (IL-8) Capture Bead B2, 13X
740707 LEGENDplex™ Human PECAM-1 Capture Bead B3, 13X
740708 LEGENDplex™ Human PlGF Capture Bead B4, 13X
740710 LEGENDplex™ Human VEGF Capture Bead B6, 13X
740711 LEGENDplex™ Human TNF-α Capture Bead B7, 13X
740712 LEGENDplex™ Human G-CSF Capture Bead B9, 13X
Selected items will be used together with:
740699 LEGENDplex™ Human Angiogenesis Panel 1 Standard
740700 LEGENDplex™ Human Angiogenesis Panel 1 Detection Antibodies
740623 LEGENDplex™ Buffer Set I
740377 LEGENDplex™ Filter Plate or 740379 LEGENDplex™ V-bottom Plate
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.