Monensin Solution (1,000X)

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Description

Monensin is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, monensin leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Monensin Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

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420701 1 ml $45.00
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Technical data sheet

Product Details

Formulation
Monensin Solution is supplied as a 1000X working solution in 70% Ethanol. Dilute to 1X in cell culture medium.
Concentration
2.0 mM
Storage & Handling
Store the solution between 2°C and 8°C. Monensin is known to be toxic; avoid direct body contact. Ethanol is flammable; keep away from sources of fire.
Application

ICFC - Quality tested

Recommended Usage

Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with monensin for ≤ 24 hours, as this can become toxic for cell viability.

Application References

(PubMed link indicates BioLegend citation)

1. Smeltz RB. 2007. J. Immunol. 178:4786.
2. Fuse S, et al. 2007. J. Immunol. 178:5227.
3. Durkin ET,et al. 2008. Blood 10:1182.PubMed
4. Durkin ET, et al. 2008. Blood 112:5245. PubMed
5. Liu XS, et al. 2009. J. Immunol. 183:51. PubMed
6. Mattarollo SR, et al. 2010. J. Immunol. 184:1242. PubMed
7. Yang M, et al. 2010. J. Immuonl. 184:3321. PubMed
8. Mattarollo SR, et al. 2010. J. Immunol. 184:5663. PubMed
9. Wood MW, et al. 2011. Infect Immun. 79:708. PubMed
10. Janune D, et al. 2011. FEBS Lett. 585:3033. PubMed
11. Fiorenza S, et al. 2012. J. Immunol. 189:5622. PubMed
12. Asgari E, et al. 2013. Blood. 122:3473. PubMed
13. Li D, et al. 2013. Clin Immunol. 149:411. PubMed
14. Bunse CE, et al. 2013. PLoS One. 8:77925. PubMed

Publication Library

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.

Gene ID
NA
UniProt
View information about Monensin Solution (1,000X) on UniProt.org

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