- Clone
- LN3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Major Histocompatibility Class II, MHC class II
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![LN3_Blue_021808 LN3_Blue_021808](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/LN3_Blue_021808.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes stained with LN3 Pacific Blue™
Cat # | Size | Price | Quantity Check Availability | Save | ||
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327016 | 100 µg | $265 |
The LN3 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- human PBL
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen sections and formalin-fixed paraffin-embedded sections1, and immunoprecipitation1.
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Application References
(PubMed link indicates BioLegend citation) -
- Marder RJ, et al. 1985. Lab. Invest. 52:497.
- Norton AJ and Isaacson PG. 1987. Am. J. Pathol. 128:225.
- Hua ZX, et al. 1998. Hum. Pathol. 29(12):1441.
- Product Citations
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- RRID
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AB_893564 (BioLegend Cat. No. 327016)
Antigen Details
- Structure
- Ig superfamily, MHC class II, heterodimeric transmembrane protein
- Distribution
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B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
- Function
- Peptide presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
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1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leuk. Biol. 48:138.
3. Edwards J, et al. 1985. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364. - Gene ID
- 3122 View all products for this Gene ID 3123 View all products for this Gene ID
- UniProt
- View information about HLA-DR on UniProt.org
Related FAQs
Other Formats
View All HLA-DR Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human HLA-DR | LN3 | FC,IHC,IP,SB |
Biotin anti-human HLA-DR | LN3 | FC,IHC |
FITC anti-human HLA-DR | LN3 | FC |
PE anti-human HLA-DR | LN3 | FC |
Alexa Fluor® 488 anti-human HLA-DR | LN3 | FC |
Alexa Fluor® 647 anti-human HLA-DR | LN3 | FC |
Alexa Fluor® 700 anti-human HLA-DR | LN3 | FC |
Pacific Blue™ anti-human HLA-DR | LN3 | FC |
PE/Cyanine7 anti-human HLA-DR | LN3 | FC |
PerCP/Cyanine5.5 anti-human HLA-DR | LN3 | FC |
APC anti-human HLA-DR | LN3 | FC |
APC/Fire™ 750 anti-human HLA-DR | LN3 | FC |
TotalSeq™-Bn1316 anti-human HLA-DR | LN3 | SB |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human HLA-DR
Human peripheral blood lymphocytes stained with purified LN3... SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
Biotin anti-human HLA-DR
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 FITC -
PE anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 PE -
Alexa Fluor® 488 anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 Alexa F... -
Alexa Fluor® 647 anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 Alexa F... -
Alexa Fluor® 700 anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN3 Alexa Fl... -
Pacific Blue™ anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN3 Pacific ... -
PE/Cyanine7 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
PerCP/Cyanine5.5 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
APC anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
APC/Fire™ 750 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
TotalSeq™-Bn1316 anti-human HLA-DR
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