- Clone
- W18021D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- L-selectin
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W18021D_Pacific-Blue_CD62L-_Antibody_022822 W18021D_Pacific-Blue_CD62L-_Antibody_022822](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W18021D_Pacific-Blue_CD62L-_Antibody_022822.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε APC and anti-mouse CD62L (clone W18021D) Pacific Blue™ (left) or rat IgG2a, κ isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
161207 | 25 µg | $94 | ||||
161208 | 100 µg | $223 |
CD62L is a 74-95 kD glycoprotein also known as L-selectin. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD62L recombinant protein (39-332)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC – Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Violet Laser (405 nm)
- RRID
-
AB_2922495 (BioLegend Cat. No. 161207)
AB_2922495 (BioLegend Cat. No. 161208)
Antigen Details
- Structure
- CD62L is a type I transmembrane glycoprotein with 74-95 kD.
- Distribution
-
CD62L is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells.
- Function
- CD62L plays roles in lymphocyte homing to lymphoid organs and inflammatory leukocyte trafficking.
- Ligand/Receptor
- CD34, GlyCAM-1, MAdCAM-1
- Cell Type
- B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Rosen S. 2004. Annu. Rev. Immunol. 22:129
2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. - Gene ID
- 20343 View all products for this Gene ID
- UniProt
- View information about CD62L on UniProt.org
Related FAQs
Other Formats
View All CD62L Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse CD62L | W18021D | FC |
Purified anti-mouse CD62L Antibody | W18021D | FC |
PE/Fire™ 810 anti-mouse CD62L | W18021D | FC |
Pacific Blue™ anti-mouse CD62L | W18021D | FC |
PerCP/Cyanine5.5 anti-mouse CD62L | W18021D | FC |
FITC anti-mouse CD62L | W18021D | FC |
PE/Cyanine7 anti-mouse CD62L | W18021D | FC |
PE/Dazzle™ 594 anti-mouse CD62L | W18021D | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse CD62L
C57BL/6 splenocytes were stained with anti-mouse CD44 PE/Cya... -
Purified anti-mouse CD62L Antibody
C57BL/6 splenocytes were stained with anti-mouse CD44 PE/Cya... -
PE/Fire™ 810 anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 F... -
Pacific Blue™ anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ... -
PerCP/Cyanine5.5 anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ... -
FITC anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ... -
PE/Cyanine7 anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ... -
PE/Dazzle™ 594 anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ...
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