PE anti-human Aiolos Antibody

Pricing & Availability
Clone
14C4C97 (See other available formats)
Other Names
IKZF3, AIO, ZNFN/A3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
14C4C97_PE_Aiolos_Antibody_1_041816
Human peripheral blood mononuclear cells were stained with CD19 APC, treated with the True-Nuclear™ Transcription Factor Buffer Set, and then stained with Aiolos (clone 14C4C97) PE (top) or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on lymphocyte population.
  • 14C4C97_PE_Aiolos_Antibody_1_041816
    Human peripheral blood mononuclear cells were stained with CD19 APC, treated with the True-Nuclear™ Transcription Factor Buffer Set, and then stained with Aiolos (clone 14C4C97) PE (top) or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on lymphocyte population.
  • 14C4C97_PE_Aiolos_Antibody_2_041816
See PE spectral data
Cat # Size Price Quantity Avail. Save
371005 25 tests $125
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371006 100 tests $315
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Description

Aiolos, also known as Ikaros family zinc finger 3 (IKZF3), is a 58kD zinc finger protein involved in hematopoetic-specific transcriptional activation. Aiolos is involved in regulation of gene expression in B lymphocytes through chromatin remodeling and formation of homodimers and heterodimers with other members of the Ikaros family. Deletion of Aiolos has been reported in pre-B-acute lymphocytic leukemia (B-ALL).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial Aiolos recombinant protein (1-130aa) expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2616803 (BioLegend Cat. No. 371005)
AB_2616804 (BioLegend Cat. No. 371006)

Antigen Details

Structure
58kD, zinc finger protein.
Distribution

T and B lymphocytes.

Function
Hematopoetic-specific transcriptional activator and chromatin remodeler.
Interaction
Forms heterodimers with Ikaros family members IKZF4 and IKZF5.
Cell Type
T cells, B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Zhuang Y, et al. 2015. Oncol. Rep. 33:1319.
2. Ma S, et al. 2008. Blood. 111:1396.
3. Billot K, et al. 2010. Leuk. Res. 34:289.
4. Morgan B, et al. 2004. EMBO J. 16:2004.
5. Wang JH, et al. 1998. Immunity. 9:543-53.
6. Hosokawa Y, et al. 1999. Genomics. 61:326.

Gene ID
22806 View all products for this Gene ID
UniProt
View information about Aiolos on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 04/20/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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