- 8e11 (See other available formats)
- Other Names
- Stage-Specific Embryonic Antigen-5
- Mouse IgG1, κ
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- Product Citations
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Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that contains the motif Fuc1-2Galβ1-3GlcNAcβ, which is characteristic of H-1 antigens. SSEA-5 is expressed on undifferentiated embryonic stem cells and pluripotent stem cells. It is developmentally regulated, and its expression is lost with cell differentiation.Product Details
- Human, African Green
- Antibody Type
- Host Species
- Human embryonic stem cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1 and immunoprecipitation1.
(PubMed link indicates BioLegend citation)
- Tang C, et al. 2011. Nat Biotechnol. 29:829. (IHC, IP)
AB_2561467 (BioLegend Cat. No. 355203)
AB_2561468 (BioLegend Cat. No. 355204)
- Glycan, H type-1 backbone, 127, and >190 kD
- Embryonic stem cells, pluripotent stem cells
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Immunology, Stem Cells
- Antigen References
1. Andrews PW. 2011. Nat. Biotechnol. 29:803.
2. Itskovitz-Eldor J. 2011. Cell. Stem. Cell. 9:291.
- Gene ID
- View information about SSEA-5 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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