- Clone
- QA18A73 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ms4a1, Ly-44
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA18A73_PE_CD20_Antibody_021622 QA18A73_PE_CD20_Antibody_021622](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA18A73_PE_CD20_Antibody_021622.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with anti-mouse/human CD45R/B220 APC and anti-mouse CD20 recombinant (clone QA18A73) PE (left) or mouse IgG2a, κ PE isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
161405 | 25 µg | $159 | ||||
161406 | 100 µg | $308 |
CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Mouse CD20 transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
This recombinant antibody clone is derived from clone SA271G2.
- RRID
-
AB_2910340 (BioLegend Cat. No. 161405)
AB_2910340 (BioLegend Cat. No. 161406)
Antigen Details
- Structure
- Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
- Distribution
-
B cells and a subset of T cells
- Function
- Regulates B-cell activation and proliferation
- Interaction
- Associates with MHC class I and II, CD53, CD81, and CD82
- Antigen References
-
1. Morsy DE, et al. 2013. J. Immunol. 191:3112.
2. Pang X, et al. 2017. Hum Gene Ther. 28(2):216-225.
3. Uchida J, et al. 2004. Int Immunol. 16(1):119-29 - Gene ID
- 12482 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD20 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD20 Recombinant Antibody | QA18A73 | FC |
APC anti-mouse CD20 Recombinant Antibody | QA18A73 | FC |
PE anti-mouse CD20 Recombinant Antibody | QA18A73 | FC |
Ultra-LEAF™ Purified anti-mouse CD20 Recombinant Antibody | QA18A73 | FC,Depletion |
FITC anti-mouse CD20 Recombinant Antibody | QA18A73 | FC |
Customers Also Purchased
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD20 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
APC anti-mouse CD20 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
PE anti-mouse CD20 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
Ultra-LEAF™ Purified anti-mouse CD20 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse/human... C57BL/6 mice were retro-orbitally injected with 200 µg of Ul... -
FITC anti-mouse CD20 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse/human...
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