- Clone
- QA20A20 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD366, Tim-3, Hepatitis A virus cellular receptor 2 homolog (HAVcr-2)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_QA20A20_PE_CD366_Antibody_1_111523 A_QA20A20_PE_CD366_Antibody_1_111523](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA20A20_PE_CD366_Antibody_1_111523.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Mouse Tim-3 transfected cells were stained with anti-mouse CD366 (Tim-3) recombinant (clone QA20A20) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). -
BALB/c splenocytes were cultured for 4 days in the presence of plate-bound purified anti-mouse CD3e, soluble purified anti-mouse CD28, and purified anti-mouse IL-4. Then cells were stained with anti-mouse CD8a APC and anti-mouse CD366 (Tim-3) (clone QA20A20) PE (right) or mouse IgG1, κ PE isotype control (left).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
165403 | 25 µg | $110 | ||||
165404 | 100 µg | $255 |
CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone QA20A20 completely blocks the binding of reference clone RMT3-23 on target cells.
- RRID
-
AB_3083286 (BioLegend Cat. No. 165403)
AB_3083286 (BioLegend Cat. No. 165404)
Antigen Details
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain with a predicted molecular weight of 31 kD; can exist as a soluble form lacking mucin and transmembrane domains.
- Distribution
-
Expressed on activated Th1 lymphocytes and CD11b+ macrophages
- Function
- May play a role in the development of immune responses and the development of Th1-mediated responses
- Interaction
- Macrophages, Th1
- Ligand/Receptor
- Putative ligand on resting CD4+ lymphocytes
- Cell Type
- Dendritic cells, Macrophages, Th1
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
- Sabatos CA, et al. 2003. Nat Immunol. 4:1102-10.
- Kuchroo VK, et al. 2003. Nat Rev Immunol. 3:454-62.
- Mooney L, et al. 2002. Nature. 415:536-41.
- Gene ID
- 171285 View all products for this Gene ID
- UniProt
- View information about CD366 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD366 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD366 (Tim-3) Recombinant Antibody | QA20A20 | FC |
PE anti-mouse CD366 (Tim-3) Recombinant Antibody | QA20A20 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD366 (Tim-3) Recombinant Antibody
Mouse Tim-3 transfected cells stained with purified anti-mou... -
PE anti-mouse CD366 (Tim-3) Recombinant Antibody
Mouse Tim-3 transfected cells were stained with anti-mouse C... BALB/c splenocytes were cultured for 4 days in the presence ...
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