PE anti-mouse CD49d Antibody

Pricing & Availability
Clone
9C10 (MFR4.B) (See other available formats)
Other Names
α4 integrin, VLA-4 α chain, integrin α4, ITGA4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
9C10_PE_090507
C57BL/6 mouse splenocytes stained with 9C10 PE
  • 9C10_PE_090507
    C57BL/6 mouse splenocytes stained with 9C10 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
103705 50 µg $105
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103706 200 µg $250
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Description

CD49d is a 150 kD glycoprotein, also known as α4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in both adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin. The 9C10(MFR4.B) antibody, in combination with the R1-2 monoclonal antibody, completely blocks VCAM-1 binding to VLA-4.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
(C57BL/6 x A/J)F1 mouse fetal liver mas
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of cell-cell adhesion and T cell costimulation1,2, and immunohistochemistry3 of acetone-fixed frozen sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103709 & 103710).

Application References

(PubMed link indicates BioLegend citation)
  1. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  2. Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
  3. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  4. Jia W, et al. 2005. Blood 106:3854. (FC)
Product Citations
  1. Simonetti G, et al. 2013. J Exp Med. 210:2887. PubMed
  2. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  3. Di C, et al. 2015. J Biol Chem. 290:12523. PubMed
  4. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  5. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  6. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  7. Valanparambil RM, et al. 2017. PLoS Pathog. 13:e1006647. PubMed
RRID
AB_313046 (BioLegend Cat. No. 103705)
AB_313047 (BioLegend Cat. No. 103706)

Antigen Details

Structure
Integrin family, associates with integrin β1 or β7, 150 kD
Distribution

T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells

Function
T cell costimulation, cell-cell and cell-matrix adhesion
Ligand/Receptor
VCAM-1, MAdCAM-1, fibronectin
Cell Type
B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994. J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.

Gene ID
16401 View all products for this Gene ID
UniProt
View information about CD49d on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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