PE anti-mouse/human Galectin-3 (Mac-2) Antibody

Pricing & Availability
Clone
Gal397 (See other available formats)
Regulatory Status
RUO
Other Names
Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
gal397_PE_071108.jpg
Human peripheral blood monocytes intracellular stained with Gal397 PE
  • gal397_PE_071108.jpg
    Human peripheral blood monocytes intracellular stained with Gal397 PE
See PE spectral data
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126706 200 µg $335
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Description

Galectin-3 (galactose-specific lectin 3) is a glycoprotein, also known as Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, and εBP. It is a member of animal β-galactoside-binding protein family that is expressed in cell nucleus, cytoplasm, plasma membrane, or extracellular matrix by variety of tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells. Galectin-3 contains carbohydrate recognition domains and binds to β-galactoside residues bearing glycoproteins, such as as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, and CD66. Galectin-3 is an adhesion molecule and plays an important role in regulation of cell proliferation, differentiation and tumor cell metastasis. Galectin-3 structurely possesses NWGR motif that is conserved in BH-1 domain of BCL-2 family and functions as an anti-apoptotic molecule.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant (partial), amino acids 151-251
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Based on immunogen information, clone Gal397 may recognize African Green Baboon and Cynomolgus. It is recommended to test the reagent for optimal performance for other species. Due to in-house testing, the purified format is not recommended for flow cytometry and intracellular flow cytometry.

Product Citations
  1. Nielsen MA, et al. 2022. Front Immunol. 13:915890. PubMed
  2. Fei F, et al. 2022. Int J Mol Sci. 23:. PubMed
  3. Zhang X, et al. 2016. J. Clin. Pathol.. 69:1100-1104. PubMed
  4. Paz H, et al. 2018. J Exp Clin Cancer Res. 37:67. PubMed
  5. Ramos-Medina R, et al. 2011. Immunol. Lett. 134:150-6. PubMed
RRID
AB_1134012 (BioLegend Cat. No. 126706)

Antigen Details

Structure
Human Galetcin-3 has 250 amino acids with a predicted molecular weight of 26 kD.
Mouse Galetcin-3 has 264 amino acids with a predicted molecular weight of 27.5 kD.
Distribution

Localized in the nucleus, cytoplasm, cell surface, or extracellular matrix. Expressed by tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, dendritic cells

Function
Regulate cell proliferation and differentiation, adhesion, anti-apoptosis
Ligand/Receptor
Variety ligands, such as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, CD66, etc
Cell Type
Dendritic cells, Epithelial cells, Macrophages, Monocytes
Biology Area
Cell Adhesion, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules
Antigen References
  1. Kim HRC, et al. 1999. Cancer Res. 59:4148
  2. Reljic R, et al. 2004. Immunol. Lett. 93:51.
  3. Takenaka Y et al. 2004. Mol. and Cell. Biol. 24:4395
  4. Joo GH, et al. 2001. J. Leukoc. Biol. 69:555
  5. Llinas L, et al. 2011. Immunol. Lett. 134:113-21
  6. Cabezón R, et al. 2011. Immunol. Lett. 134:167-73
Gene ID
16854 View all products for this Gene ID 3958 View all products for this Gene ID
UniProt
View information about Mac-2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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