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PE anti-mouse Isthmin-1 Antibody

Pricing & Availability
Clone
B054B6 (See other available formats)
Regulatory Status
RUO
Other Names
Ism1, BATAM
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
B054B6_PE_Isthmin-1 _Antibody_IHC_093013.jpg
HEK-293 cells transfected with murine Isthmin-1 were fixed, permeabilized, and then stained with clone B054B6 PE (filled histogram) or rat IgG2a, κ isotype control PE (open histogram).
  • B054B6_PE_Isthmin-1 _Antibody_IHC_093013.jpg
    HEK-293 cells transfected with murine Isthmin-1 were fixed, permeabilized, and then stained with clone B054B6 PE (filled histogram) or rat IgG2a, κ isotype control PE (open histogram).
See PE spectral data
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145003 25 µg $160
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Description

Isthmin is a secreted protein, and it was initially identified in the Xenopus midbrain-hindbrain organizer (MHB) or isthmus organizer, where it is highly expressed. The MHB is an important signaling center in vertebrates and regulates the polarized morphological differentiation of the adjacent tectum and cerebellum. Mouse Isthmin is a 454 amino acid protein containing a Thrombospondin Type 1 Repeat (TSR) domain in the central region and an Adhesion-associated domain in MUC4 and Other Proteins (AMOP) domain at the C-terminal. The TSR domains are highly conserved with 98% identity between mouse and human; 87-88% identity between mouse and Xenopus. The C-terminal AMOP domains are also highly conserved with 99% identity between mouse and human; 91% identity between mouse and Xenopus. Mouse isthmin nucleotide sequence is similar to the human chromosome 20 open reading frame 82 (C20orf82). The Ism1 gene is conserved in human, chimpanzee, dog, cow, chicken, and zebrafish. Isthmin has angiostatic activity in vitro and in vivo. Isthmin inhibits EC capillary network formation mainly by interfering with the early stages of in vitro angiogenesis on matrigel. Isthmin inhibits VEGF-induced EC proliferation and induces apoptosis in the presence of VEGF. Overexpression of isthmin in B16 melanoma inhibits tumor growth and tumor angiogenesis in mice. Knockdown of isthmin in zebrafish embryos leads to abnormal intersegmental vessel (ISV) formation in the trunk.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length mouse Isthmin 1 recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2562584 (BioLegend Cat. No. 145003)

Antigen Details

Structure
454 aa with a predicted molecular weight of 51 kD.
Distribution

Isthmin is highly expressed in the midbrain-hindbrain organizer.

Interaction
Isthmin interacts with endothelial cells.
Biology Area
Cell Biology, Signal Transduction, Ubiquitin/Protein Degradation
Antigen References

1. Pera EM, et al. 2002. Mech. Dev. 116:169.
2. Xiang W, et al. 2009. J. Cell. Mol. Med. 15:359.
3. Zhang Y, et al. 2011. Cell Death. Dis. 2:e153.

Gene ID
319909 View all products for this Gene ID
UniProt
View information about Isthmin-1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 10/03/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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