PE anti-mouse TCR Vβ11 Antibody

Pricing & Availability
Clone
KT11 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vβ 11 [vb11]
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
KT11_PE_1_072811
C57BL/6 splenocytes were stained with CD3 APC and TCR Vβ11 (clone KT11) PE (top) or rat IgG2b, κ PE isotype control (bottom).
  • KT11_PE_1_072811
    C57BL/6 splenocytes were stained with CD3 APC and TCR Vβ11 (clone KT11) PE (top) or rat IgG2b, κ PE isotype control (bottom).
  • KT11_PE_2_072811
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125907 100 µg $238
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Description

The Vβ11 gene locus is deleted in mice of TCR a haplotype (C57BR, C57L, SJL, SWR) and TCR c haplotype (RIII).Mice of TCR b haplotype (C57BL, BALB/c, AKR, etc) have Vβ11-bearing T cells .However, mice expressing MHC class II I-E and some superantigens, such as Mtv-8, Mtv-9 and Mtv-11 will show a partial deletion of Tb11-bearing T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Quigley MF, et al. 2010. Proc Natl Acad Sci USA. 9:19414. PubMed
Product Citations
  1. Ohkusu-Tsukada K, et al. 2017. Biosci Biotechnol Biochem. 2160:81. PubMed
  2. Valentine KM, et al. 2018. J Immunol. 31:201. PubMed
  3. Moorman CD, et al. 2019. Front Immunol. 3119:9. PubMed
  4. Quigley M, et al. 2010. Proc Natl Acad Sci U S A. 107:19414. PubMed
  5. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
RRID
AB_1227781 (BioLegend Cat. No. 125907)

Antigen Details

Distribution

Positive on a subset of alpha/beta T cells

Ligand/Receptor
MHC/peptide
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR Vbeta11 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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