- Clone
- A3C6E2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HLDA6
- Other Names
- Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A3C6_PECy7_033007 A3C6_PECy7_033007](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A3C6_PECy7_033007.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human erythroleukemia cell line TF-1 stained with A3C6 PE/Cyanine7
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
323414 | 100 tests | $323 |
CD117 is a 145 kD protein tyrosine kinase also known as c-kit. It is a receptor for stem cell factor or c-kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemic cells (AML). CD117 binding of c-kit ligand induces phosphorylation of CD117, and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells. The A3C6E2 antibody potently blocks the binding of SCF.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- MOLM-1 megakaryocitic cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: Blocking the binding of SCF. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 323417 & 323418).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- RRID
-
AB_755984 (BioLegend Cat. No. 323414)
Antigen Details
- Structure
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
- Distribution
-
Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Function
- Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decre
- Ligand/Receptor
- c-Kit ligand
- Modification
- Multiple phosphorylation sites
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736. - Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
Related FAQs
Other Formats
View All CD117 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD117 (c-kit) | A3C6E2 | FC |
Biotin anti-human CD117 (c-kit) | A3C6E2 | FC |
PE anti-human CD117 (c-kit) | A3C6E2 | FC |
PE/Cyanine5 anti-human CD117 (c-kit) | A3C6E2 | FC |
PE/Cyanine7 anti-human CD117 (c-kit) | A3C6E2 | FC |
PerCP/Cyanine5.5 anti-human CD117 (c-kit) | A3C6E2 | FC |
Ultra-LEAF™ Purified anti-human CD117 (c-kit) | A3C6E2 | FC,Block |
TotalSeq™-D1260 anti-human CD117 (c-kit) | A3C6E2 | PG |
Customers Also Purchased
![](/Files//Images/media_assets/products/product_images/ACFiPjs0M.jpg)
![](/Files//Images/media_assets/products/product_images/HIB19_FITC_061807.jpg)
![](/Files//Images/media_assets/products/product_images/HI10a_FITC_061807.jpg)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD117 (c-kit)
Human erythroleukemic cell line TF-1 stained with purified A... -
Biotin anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with biotinylat... -
PE anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with A3C6 PE -
PE/Cyanine5 anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with A3C6 PE/Cy... -
PE/Cyanine7 anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with A3C6 PE/Cy... -
PerCP/Cyanine5.5 anti-human CD117 (c-kit)
Human erythroleukemia cell line, HEL, was stained with CD117... -
Ultra-LEAF™ Purified anti-human CD117 (c-kit)
Human erythroleukemic cell line TF-1 stained with Ultra-LEAF... -
TotalSeq™-D1260 anti-human CD117 (c-kit)
Follow Us