- Clone
- S20019D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- interleukin-2 receptor alpha chain, interluekin-2 receptor, IL2RA, IL-2Rα
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S20019D_PECyanine7_CD25_Antibody_1_062423 S20019D_PECyanine7_CD25_Antibody_1_062423](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S20019D_PECyanine7_CD25_Antibody_1_062423.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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PHA-activated human peripheral blood lymphocytes were stained with anti-human CD25 (clone S20019D) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram). -
Human peripheral blood lymphocytes were stained with anti-human CD25 (clone S20019D) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). Cells were costained with anti-human CD4 APC.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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385203 | 25 tests | $145 | ||||
385204 | 100 tests | $315 |
CD25 is a 55 kD transmembrane protein also known as interleukin-2 receptor alpha chain. CD25 in combination with CD122 and CD132 form a high affinity IL-2 receptor that binds to IL-2, playing a crucial role in activation of JAK1/JAK3 and STAT5 signaling. It is expressed on activated T cells as well as on CD4+ T regulatory cells, some progenitor B-cells and activated monocytes.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CD25 transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.
Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.
Clone S20019D does not affect binding of IL-2 to the receptor.
Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed. - RRID
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AB_3068149 (BioLegend Cat. No. 385203)
AB_3068149 (BioLegend Cat. No. 385204)
Antigen Details
- Structure
- 55 kD transmembrane protein
- Distribution
-
Activated T cells, T regulatory cells, monocytes
- Function
- IL-2 receptor
- Interaction
- CD122, CD132
- Ligand/Receptor
- IL-2
- Cell Type
- Lymphocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Waldmann T. 1991. J Biol Chem. 266:2681-4.
- Sakaguchi S, et al. 1995. The Journal of Immunology. 155:1151-64.
- Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Other Formats
View All CD25 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD25 | S20019D | FC |
PE/Cyanine7 anti-human CD25 | S20019D | FC |
APC anti-human CD25 | S20019D | FC |
Brilliant Violet 421™ anti-human CD25 | S20019D | FC |
PE anti-human CD25 | S20019D | FC |
Spark Red™ 718 anti-human CD25 | S20019D | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD25
PHA-activated human peripheral blood lymphocytes were staine... Human peripheral blood lymphocytes were stained with purifie... -
PE/Cyanine7 anti-human CD25
PHA-activated human peripheral blood lymphocytes were staine... Human peripheral blood lymphocytes were stained with anti-hu... -
APC anti-human CD25
PHA-activated human peripheral blood lymphocytes were staine... Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 421™ anti-human CD25
PHA-activated human peripheral blood lymphocytes were stain... Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD25
HA-activated human peripheral blood lymphocytes were stained... Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD25
Human peripheral blood lymphocytes were stained with anti-hu...
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