- Clone
- 281-2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Syndecan-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![281-2_PECy7_CD138_Antibody_FC_1_060413 281-2_PECy7_CD138_Antibody_FC_1_060413](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/281-2_PECy7_CD138_Antibody_FC_1_060413.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD19 APC and CD138 (clone 281-2) PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
142513 | 25 µg | $176 | ||||
142514 | 100 µg | $382 |
CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse mammary gland epithelial cell line NMuMG
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
- Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
- Li Q, et al. 2002. Cell 111:635. (IF, IHC)
- McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
- Product Citations
-
- RRID
-
AB_2562197 (BioLegend Cat. No. 142513)
AB_2562198 (BioLegend Cat. No. 142514)
Antigen Details
- Structure
- Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
- Function
- Cell adhesion, cell motility, cell migration, cell proliferation
- Cell Type
- B cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27. - Gene ID
- 20969 View all products for this Gene ID
- UniProt
- View information about CD138 on UniProt.org
Other Formats
View All CD138 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC ... -
PE anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC ... -
APC anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC ... -
Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 APC a... -
PerCP/Cyanine5.5 anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC ... -
Biotin anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse splenocytes were stained with CD19 APC and bio... -
PE/Cyanine7 anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse splenocytes were stained with CD19 APC and CD1... -
Brilliant Violet 605™ anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 APC a... -
Brilliant Violet 650™ anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC ... -
Brilliant Violet 711™ anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse splenocytes were stained with CD19 APC and CD1... -
Brilliant Violet 510™ anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 APC a... -
Alexa Fluor® 647 anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC ... -
PE/Dazzle™ 594 anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 APC a... -
APC/Cyanine7 anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC ... -
TotalSeq™-A0810 anti-mouse CD138 (Syndecan-1)
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Brilliant Violet 785™ anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with CD19 FITC ... -
TotalSeq™-C0810 anti-mouse CD138 (Syndecan-1)
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TotalSeq™-B0810 anti-mouse CD138 (Syndecan-1)
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PE/Cyanine5 anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
APC/Fire™ 750 anti-mouse CD138 (Syndecan-1)
C577BL/6 mouse bone marrow cells were stained with anti-mous... -
PE/Fire™ 640 anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
PE/Fire™ 810 anti-mouse CD138 (Syndecan-1)
C57BL/6 mouse bone marrow cells were stained with anti-mouse...
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