- Clone
- SF1-1.1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class I
- Isotype
- Mouse (SJL) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![SF1-1.1_PECy7_H-2Kd_ANtibody_FC_111313.jpg SF1-1.1_PECy7_H-2Kd_ANtibody_FC_111313.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/SF1-1.1_PECy7_H-2Kd_ANtibody_FC_111313.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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BALB/c splenocytes were stained with H-2Kd (clone SF1-1.1) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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116621 | 25 µg | $124 | ||||
116622 | 100 µg | $323 |
The SF1-1.1 antibody reacts with the H-2Kd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd haplotype. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- BALB/c Mouse cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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The SF1-1.1 antibody is weakly cross-reactive with H-2k but does not cross-react with other haplotypes (b, j, p, q, s, v). Clone SF1-1.1 recognizes the a3 domain of Kd.
Additional reported applications (for the relevant formats) include: immunoprecipitation1,4 and Western blotting2. - Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Noun G, et al. 1996. J. Immunol. 157:2455. (IP)
- Abastado JP, et al. 1993. J. Immunol. 151:3569. (WB)
- Bashuda H, et al. 1997. Transplantation 63:113.
- Sester M, et al. 2000. J. Biol. Chem. 34:113. (IP)
- Ma XT, et al. 2006. Cancer Res. 66:1169. (FC)
- Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
- Norian L, et al. 2004. J. Immunol.. 173:835. PubMed
- Delon J, et al. 1998. Immunity 9:467.
- Product Citations
-
- RRID
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AB_2562732 (BioLegend Cat. No. 116621)
AB_2562733 (BioLegend Cat. No. 116622)
Antigen Details
- Structure
- MHC class I
- Distribution
-
Nucleated cells of the H-2Kd haplotype
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
- Watts C. 1997. Annu. Rev. Immunol. 15:821.
- Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
- York I, et al. 1996. Annu. Rev. Immunol. 14:369.
- Gene ID
- 14972 View all products for this Gene ID
- UniProt
- View information about H-2Kd on UniProt.org
Related FAQs
Other Formats
View All H-2Kd Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse H-2Kd | SF1-1.1 | FC |
FITC anti-mouse H-2Kd | SF1-1.1 | FC |
PE anti-mouse H-2Kd | SF1-1.1 | FC |
Purified anti-mouse H-2Kd | SF1-1.1 | FC,IP,WB |
Alexa Fluor® 488 anti-mouse H-2Kd | SF1-1.1 | FC |
Alexa Fluor® 647 anti-mouse H-2Kd | SF1-1.1 | FC |
Pacific Blue™ anti-mouse H-2Kd | SF1-1.1 | FC |
PerCP/Cyanine5.5 anti-mouse H-2Kd | SF1-1.1 | FC |
APC anti-mouse H-2Kd | SF1-1.1 | FC |
PE/Cyanine7 anti-mouse H-2Kd | SF1-1.1 | FC |
Brilliant Violet 421™ anti-mouse H-2Kd | SF1-1.1 | FC |
Brilliant Violet 510™ anti-mouse H-2Kd | SF1-1.1 | FC |
Alexa Fluor® 700 anti-mouse H-2Kd | SF1-1.1 | FC |
APC/Cyanine7 anti-mouse H-2Kd | SF1-1.1 | FC |
PE/Dazzle™ 594 anti-mouse H-2Kd | SF1-1.1 | FC |
Brilliant Violet 605™ anti-mouse H-2Kd | SF1-1.1 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse H-2Kd
Balb/c mouse splenocytes stained with biotinylated SF1-1.1, ... -
FITC anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 FITC -
PE anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 PE -
Purified anti-mouse H-2Kd
Balb/c mouse splenocytes stained with purified SF1-1.1, foll... Balb/c mouse splenocytes stained with purified SF1-1.1, foll... -
Alexa Fluor® 488 anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 Alexa Fluor&re... -
Alexa Fluor® 647 anti-mouse H-2Kd
BALB/c mouse splenocytes stained with SF1-1.1 Alexa Fluor&re... -
Pacific Blue™ anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 Pacific Blue&t... C57BL/6 mouse splenocytes stained with SF1-1.1 Pacific Blue&... -
PerCP/Cyanine5.5 anti-mouse H-2Kd
BALB/c mouse splenocytes were stained with H-2Kd ... -
APC anti-mouse H-2Kd
BALB/c splenocytes stained with SF1-1.1 APC -
PE/Cyanine7 anti-mouse H-2Kd
BALB/c splenocytes were stained with H-2Kd (clone... -
Brilliant Violet 421™ anti-mouse H-2Kd
BALB/c mouse splenocytes were stained with H-2Kd ... -
Brilliant Violet 510™ anti-mouse H-2Kd
BALB/c mouse splenocytes were stained with H-2Kd ... -
Alexa Fluor® 700 anti-mouse H-2Kd
BALB/c mouse splenocytes were stained with H-2Kd ... -
APC/Cyanine7 anti-mouse H-2Kd
BALB/c mouse splenocytes were stained with H-2Kd ... -
PE/Dazzle™ 594 anti-mouse H-2Kd
BALB/c splenocytes were stained with H-2Kd (clone... -
Brilliant Violet 605™ anti-mouse H-2Kd
BALB/c splenocytes were stained with H-2Kd (clone...
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