- Clone
- S18009F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T200, Ly-5, LCA
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S18009F_PEFire810_CD45_Antibody_080621.png S18009F_PEFire810_CD45_Antibody_080621.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S18009F_PEFire810_CD45_Antibody_080621.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes were stained with anti-mouse CD45 (clone S18009F) PE/Fire™ 810 (filled histogram) or left unstained (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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157215 | 25 µg | $176 |
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD45
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page. - Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2894659 (BioLegend Cat. No. 157215)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets
- Function
- Phosphatase, T and B cell activation
- Interaction
- Mesenchymal Stem Cells, B cells, Dendritic cells, Tregs
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
- Biology Area
- Cell Biology, Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Trowbridge IS, et al. 1994. Annu. Rev. Immunol. 12:85.
- Kishihara K, et al. 1993. Cell. 74:143.
- Pulido R, et al. 1988. J. Immunol. 140:3851.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
Related FAQs
Other Formats
View All CD45 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD45 | S18009F | FC |
APC/Cyanine7 anti-mouse CD45 | S18009F | FC |
PE/Cyanine7 anti-mouse CD45 | S18009F | FC |
PerCP/Cyanine5.5 anti-mouse CD45 | S18009F | FC |
Alexa Fluor® 700 anti-mouse CD45 | S18009F | FC |
Pacific Blue™ anti-mouse CD45 | S18009F | FC |
PE/Fire™ 810 anti-mouse CD45 | S18009F | FC |
FITC anti-mouse CD45 | S18009F | FC |
Brilliant Violet 605™ anti-mouse CD45 | S18009F | FC |
Brilliant Violet 510™ anti-mouse CD45 | S18009F | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD45
C57BL/6 splenocytes were stained with purified CD45 (clone S... -
APC/Cyanine7 anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone S18009F) A... -
PE/Cyanine7 anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone S18009F) P... -
PerCP/Cyanine5.5 anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone S18009F) P... -
Alexa Fluor® 700 anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone S18009F) A... -
Pacific Blue™ anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone S18009F) P... -
PE/Fire™ 810 anti-mouse CD45
C57BL/6 splenocytes were stained with anti-mouse CD45 (clone... -
FITC anti-mouse CD45
C57BL/6 mouse splenocytes were stained with anti-mouse CD45 ... -
Brilliant Violet 605™ anti-mouse CD45
C57BL/6 splenocytes were stained with anti-mouse CD45 (clone... -
Brilliant Violet 510™ anti-mouse CD45
C57BL/6 mouse splenocytes were stained with anti-mouse CD45 ...
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