- Clone
- AF6-88.5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class I
- Isotype
- Mouse (BALB/c) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 splenocytes stained with AF6-88.5 PerCP/Cyanine5.5
Cat # | Size | Price | Quantity Check Availability | Save | ||
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116515 | 25 µg | $141 | ||||
116516 | 100 µg | $347 |
The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C57BL mouse splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.
Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v). - Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
- Wall KA, et al. 1983. J. Immunol. 131:1056.
- Andersson M, et al. 1998. J. Immunol. 161:6475.
- Shao H, et al. 2005. J. Immunol. 175:1851.
- Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
- Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
- Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
- Product Citations
-
- RRID
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AB_1967107 (BioLegend Cat. No. 116515)
AB_1967133 (BioLegend Cat. No. 116516)
Antigen Details
- Structure
- MHC class I
- Distribution
-
Nucleated cells of the H-2Kb haplotype
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
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1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369. - Gene ID
- 14972 View all products for this Gene ID
- UniProt
- View information about H-2Kb on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All H-2Kb Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse H-2Kb | AF6-88.5 | FC |
FITC anti-mouse H-2Kb | AF6-88.5 | FC |
PE anti-mouse H-2Kb | AF6-88.5 | FC |
Purified anti-mouse H-2Kb | AF6-88.5 | FC,IHC-F,IP |
Alexa Fluor® 488 anti-mouse H-2Kb | AF6-88.5 | FC |
Alexa Fluor® 647 anti-mouse H-2Kb | AF6-88.5 | FC |
Pacific Blue™ anti-mouse H-2Kb | AF6-88.5 | FC |
PerCP/Cyanine5.5 anti-mouse H-2Kb | AF6-88.5 | FC |
APC anti-mouse H-2Kb | AF6-88.5 | FC |
PE/Cyanine7 anti-mouse H-2Kb | AF6-88.5 | FC |
Alexa Fluor® 700 anti-mouse H-2Kb | AF6-88.5 | FC |
Brilliant Violet 510™ anti-mouse H-2Kb | AF6-88.5 | FC |
Brilliant Violet 421™ anti-mouse H-2Kb | AF6-88.5 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with biotinylated AF6-88.5... -
FITC anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with AF6-88.5 FITC -
PE anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with AF6-88.5 PE -
Purified anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with purified AF6-88.5, fo... -
Alexa Fluor® 488 anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with AF6-88.5 Alexa Fluor®... -
Alexa Fluor® 647 anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with AF6-88.5 Alexa Fluor®... -
Pacific Blue™ anti-mouse H-2Kb
C57BL/6 splenocytes stained with AF6-88.5 Pacific Blue&trade... -
PerCP/Cyanine5.5 anti-mouse H-2Kb
C57BL/6 splenocytes stained with AF6-88.5 PerCP/Cyanine5.5 -
APC anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with AF6-88.5 APC -
PE/Cyanine7 anti-mouse H-2Kb
C57BL/6 mouse splenocytes were stained with PE/Cyanine7 AF6-... -
Alexa Fluor® 700 anti-mouse H-2Kb
C57BL/6 mouse spenocyte were stained with H-2Kb (... -
Brilliant Violet 510™ anti-mouse H-2Kb
C57BL/6 mouse splenocytes were stained with H-2Kb (clone AF6... -
Brilliant Violet 421™ anti-mouse H-2Kb
C57BL/6 mouse splenocytes were stained with H-2Kb...
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