- Clone
- J252D4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BLR1, MDR15
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![/J252D4_PERCEP-FIRE-780_CD185_Antibody_111023 /J252D4_PERCEP-FIRE-780_CD185_Antibody_111023](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/J252D4_PERCEP-FIRE-780_CD185_Antibody_111023-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human CD19 APC and anti-human CD185 (CXCR5) (clone J252D4) PerCP/Fire™ 780 (left) or mouse IgG1, κ PerCP/Fire™ 780 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
356959 | 25 tests | $188 | ||||
356960 | 100 tests | $440 |
CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CXCR5-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 780 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Fire 780 has a maximum excitation of 478 nm and a maximum emission of 780 nm. - Excitation Laser
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Blue Laser (488 nm)
- RRID
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AB_3097330 (BioLegend Cat. No. 356959)
AB_3097330 (BioLegend Cat. No. 356960)
Antigen Details
- Structure
- G-protein coupled receptor, 7 transmembrane regions, 42 kD
- Distribution
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Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.
- Function
- Mediates cell migration to the B cell follicles in the secondary lymphoid organs
- Ligand/Receptor
- CXCL13
- Cell Type
- B cells, Tfh
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
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1. Ma CS, et al. 2012. J. Exp. Med. 209:1241.
2. León B, et al. 2012. Nat. Immunol. 13:681.
3. Crotty S. 2011. Annu. Rev. Immunol. 29:621.
4. Kerfoot SM, et al. 2011. Immunity 34:947.
5. Sáez de Guinoa J, et al. 2011. Blood 118:1560. - Gene ID
- 643 View all products for this Gene ID
- UniProt
- View information about CD185 on UniProt.org
Related FAQs
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
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Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
Other Formats
View All CD185 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with anti-hu... -
APC anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
Purified anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 647 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
PerCP/Cyanine5.5 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
Alexa Fluor® 488 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
FITC anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
Alexa Fluor® 700 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
Pacific Blue™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
Brilliant Violet 421™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 Al... -
PE/Cyanine7 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
APC/Cyanine7 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
PE/Dazzle™ 594 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
Brilliant Violet 605™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
Biotin anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 711™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 785™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 AP... -
TotalSeq™-A0144 anti-human CD185 (CXCR5)
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TotalSeq™-C0144 anti-human CD185 (CXCR5)
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Brilliant Violet 750™ anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with CD19 FI... -
TotalSeq™-B0144 anti-human CD185 (CXCR5)
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APC/Fire™ 750 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes stained with CD19 Pacific... -
Spark NIR™ 685 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes stained with CD19 PE and ... -
TotalSeq™-D0144 anti-human CD185 (CXCR5)
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PE/Cyanine5 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human CD185 (CXCR5) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 810 anti-human CD185 (CXCR5) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 806 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 780 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes were stained with anti-hu...
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