- 3A9 (See other available formats)
- HCDM listed
- Other Names
- PDCD6IP, PDCD6 interacting protein, AIP1, Hp95
- Mouse IgG1, κ
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- Product Citations
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Alix (ALG-2-interacting protein X) is an adaptor protein that was first described for its capacity to bind to the calcium-binding protein ALG-2, the expression of which seemed necessary for cell death. The predicted molecular weight is approximately 96 kD. Alix contains an N-terminal Bro1 domain and a C-terminal proline-rich domain (PRD). The PRD contains multiple polyproline motifs, which are potential docking sites for proteins containing SH3 domains, interact with multiple cellular Alix-binding partners that are involved in apoptotic induction, endosomal sorting, and endocytosis. The Bro1 domain interacts with CHMP4b, which is also involved in endosomal sorting.Product Details
- Human, Mouse, and Frog
- Antibody Type
- Host Species
- Recombinant full-length human Alix protein
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC, IP - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone 3A9 has been shown to be useful for Western blotting1, immunoprecipitation, and immunofluorescence2 staining of the human and mouse Alix protein.
Clone 3A9 shows strong specificity for mouse Alix.
(PubMed link indicates BioLegend citation)
- Product Citations
AB_2268110 (BioLegend Cat. No. 634501)
AB_2162471 (BioLegend Cat. No. 634502)
- Belongs to a conserved family of proteins that have in common an N-terminal Bro1 domain and a C-terminal proline-rich domain. Predicted molecular weight approximately 96 kD.
Cytoplasm. Alix is also abundant within extracellular vesicles known as exosomes, and enriched in virions that are released from cells under the direction of the HIV-1 Gag protein.
- Adaptor in signal transduction pathways. Involved in apoptosis and endosomal membrane trafficking and viral budding.
- ALG2, SH3GL2, H-Vps28, CHMP4B, CHMP4A, CHMP4C, SETA, CIN85, Src
- Biology Area
- Cell Biology
- Antigen References
1. Wu Y, et al. 2000. Differentiation 67:139.
2. Missotten M, et al. 1999. Cell Death Differ. 6:124.
3. Odorizzi G. 2006. J Cell Sci. 119:3025.
- Gene ID
- 10015 View all products for this Gene ID
- View information about Alix on UniProt.org
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