- Clone
- 9E11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- myc tag, c-Myc tag, cMyc tag, Myc epitope tag
- Previously
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Covance Catalog# MMS-164P
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![9E11_PURE_c-Myc_Antibody_1_071921 9E11_PURE_c-Myc_Antibody_1_071921](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9E11_PURE_c-Myc_Antibody_1_071921.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from HeLa cells mixed with the indicated amount of recombinant Posi-tag epitope tag protein were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-c-Myc antibody (clone 9E11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
ICC staining of purified anti-c-Myc antibody (clone 9E11) on HeLa cells transfected with multi-tag plasmid containing c-Myc epitope sequence. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100, and blocked with 5% fetal bovine serum in PBS for 1 hour. The cells were then incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 goat anti-mouse IgG (green) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20X objective. Scale bar: 100 µm -
IHC staining of anti-c-Myc antibody (clone 9E11) on formalin-fixed paraffin-embedded human bladder tissue. Following antigen retrieval using 0.1M Tris-buffered saline w/ Tween 20 (Cat. No. 925501), the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong&reade; Gold Antifade Mountant. The image was captured with a 20x objective. Scale bar: 100 µm -
Whole cell extracts (250 µg total protein) prepared from HeLa cells mock-transfected (-) or transfected (+) with multi-tag plasmid containing c-Myc epitope sequence were immunoprecipitated overnight with 2.5 µg of purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400101) or purified anti-c-Myc antibody (clone 9E11). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with purified anti-c-Myc antibody (clone 9E11) at 1.0 µg/mL (1:1000 dilution). Lane M: Molecular weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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904401 | 100 µL | $356 |
c-Myc truncations are often used as an epitope tag in order to simplify the labeling and detection of proteins. This antibody recognizes the c-Myc epitope tag and fused recombinant proteins allowing for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.
Product DetailsProduct Details
- Verified Reactivity
- All Species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
ICC, IHC-P, IP - Verified
Purification - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The 9E11 monoclonal antibody recognizes the sequence 408-420 AEEQKLISEEDL of human c-myc.
This clone recognizes a non-specific band in BL21 E. coli lysates
BioLegend offers two clones against c-Myc, 9E10 and 9E11. When both clones were tested in parallel for WB, ICC, and IP, each clone performed similarly, with 9E11 showing slightly better performance for each application. - Additional Product Notes
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This antibody recognizes endogenous human c-Myc protein in IHC-P. Reactivity with endogenous c-Myc in other applications is not verified.
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Application References
(PubMed link indicates BioLegend citation) -
- Halme A, et al. 2004. Cell. 116:405.
- Kolodziej P, Young R. 1991. Meth. Enzymol. 194:508.
- Locker AP, et al. 1989. Brit. J. Cancer. 60:669.
- Product Citations
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- RRID
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AB_2565039 (BioLegend Cat. No. 904401)
Antigen Details
- Biology Area
- Cell Biology, Protein Purification, Protein Synthesis
- Gene ID
- 4609 View all products for this Gene ID
- UniProt
- View information about c-Myc on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All c-Myc Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-c-Myc | 9E11 | WB,ICC,IHC-P,IP,Purification |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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