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Purified anti-CD54 (ICAM-1) Antibody

Pricing & Availability
Clone
W21128C (See other available formats)
Regulatory Status
RUO
Other Names
CD54, ICAM-1, Intercellular Adhesion Molecule 1, Cluster of Differentiation 54
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
a. W21128C_PURE_CD54_ICC_1_030824
A549 cells without (panel A) or treated with 10 ng/mL of recombinant human TNF-α (Cat. No. 570104) (panel B) for 6 hours, were fixed with fixation buffer (Cat. 420801) for 10 minutes, permeabilized with 0.5% Triton X-100, and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5 µg/mL of Purified anti-CD54 (ICAM-1) (clone W21128C), followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 Goat anti-rat IgG (Cat. No. 405418) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). The image was captured with a 40X objective. Scale bar: 25 µm
  • a. W21128C_PURE_CD54_ICC_1_030824
    A549 cells without (panel A) or treated with 10 ng/mL of recombinant human TNF-α (Cat. No. 570104) (panel B) for 6 hours, were fixed with fixation buffer (Cat. 420801) for 10 minutes, permeabilized with 0.5% Triton X-100, and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5 µg/mL of Purified anti-CD54 (ICAM-1) (clone W21128C), followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 Goat anti-rat IgG (Cat. No. 405418) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). The image was captured with a 40X objective. Scale bar: 25 µm
  • b. W21128C_PURE_CD54_ICC_2_030824
    Raji cells were fixed with Fixation Buffer (Cat. 420801) for 10 minutes, permeabilized with 0.5% Triton X-100, and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5 µg/mL of Purified anti-CD54 (ICAM-1) (clone W21128C), followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 Goat anti-rat IgG (Cat. No. 405418) for 1 hour at room temperature (panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (panel B, blue). The image was captured with a 40X objective. Scale bar: 25 µm
  • c. W21128C_PURE_CD54_Direct-ELISA_3_030824
    Direct ELISA showing Purified anti-CD54 (ICAM-1) (clone W21128C) bound to plate-immobilized recombinant human ICAM-1-Fc Chimera protein (Cat. Nos. 552904 and 552906). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA in PBS, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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636351 25 µg $170
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636352 100 µg $445
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Description

Intercellular adhesion molecule 1, also known as ICAM-1 or CD54, is a member of the immunoglobulin superfamily. Structurally, ICAM-1 is characterized by heavy glycosylation with an extracellular domain composed of multiple loops created by disulfide bridges within the protein. Beta sheets are the dominant secondary structure of this protein, suggesting the presence of dimerization domains in ICAM-1. This protein is constitutively expressed at low levels on the cell surface of leukocytes, endothelial cells, macrophages, and lymphocytes. Upon cytokine activation, by cytokines such as IL-1 and TNFα, the concentration of ICAM-1 on the cell surface greatly increases. The ligand of ICAM-1 is LFA-1 (leukocyte function associated antigen 1), which is found on all T cells. LFA-1/ICAM-1 interaction has been shown to be important for T cell-T cell interactions, which leads to further T cell differentiation. ICAM-1 was found to play a role in infectious diseases, as it was identified as the binding target for entry of the major group of human rhinoviruses into various cell types and is also known for its affinity for Plasmodium falciparum-infected erythrocytes. These findings indicate that ICAM-1 may participate in the pathogenesis of several infectious agents.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Extracellular domain of recombinant human ICAM-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For Direct ELISA, the optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice-cold methanol or fixation with ice-cold methanol is recommended.

Antigen Details

Structure
ICAM-1 protein is a 532 amino acid protein with a predicted molecular weight of 58 kD.
Distribution

Endothelial cells, T cells and B cells, monocytes/macrophages, granulocytes, and dendritic cells

Function
Cellular adhesion, involved in inflammation and immune reactions. CD54 expressed on endothelial cells in areas of inflammation plays a role in leukocyte extravasation. Major cellular receptor for rhinovirus.
Interaction
MUC1 and promotes cell aggregation in epithelial cells, ARHGEF26/SGEF, (on T cell side) with CD81, CD247 and CD9 at immunological synapses between antigen-presenting cells and T cells, major receptor group rhinovirus A-B capsid proteins, and Coxsackievirus A21 capsid proteins.
Ligand/Receptor
CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) and has also been reported to bind hyaluronan and fibrinogen
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Bella J, et al. 1998. Proc Natl Acad Sci USA. 95:4140-5.
  2. Katagiri T, et al 1996. Blood. 87:4276-85.
  3. Rothlein RD. 1986. J Immunol. 137:1270.
  4. Yang L, et al. 2005. Blood. 106:584-92.
  5. Voraberger G, et al. 1991. J Immunol. 147:2777-86.
  6. Staunton DE, et al. 1988. Cell. 52:925-33.
  7. Greve JM, et al. 1989. Cell. 56:839-47.
  8. van Buul JD, et al. 2010. PLoS One. 5:e11336.
Gene ID
3383 View all products for this Gene ID
UniProt
View information about CD54 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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