- Clone
- DA-7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Keratin 18, Keratin type I cytoskeletal 18
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![DA-7_pure_cytokeratin18_Antibody_1_062518 DA-7_pure_cytokeratin18_Antibody_1_062518](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/DA-7_pure_cytokeratin18_Antibody_1_062518.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell lysates (15 µg protein) from A549, A431, HeLa, HEL (negative control) and U87-MG (negative control) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-Cytokeratin 18 antibody, clone DA-7. Proteins were visualized using chemiluminescence detection by incubating the membrane with a 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Cell lysates were loaded in order of descending KRT18 mRNA expression levels (source: Human Protein Atlas). Lane M: Molecular Weight Marker. -
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-cytokeratin 18 antibody (clone DA-7). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. -
MCF-7 cells were stained with anti-Cytokeratin 18 (clone DA-7), followed by Alexa Fluor® 546 secondary antibody and DAPI (nuclei). Images were aquired on a Nikon FC300 inverted microscope at 20X magnification. Data provided by Dr. John Nolan, La Jolla Bioengineering Institute.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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628401 | 25 µg | $101 | ||||
628402 | 100 µg | $218 |
Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein with a molecular weight of approximately 48 kD. Cytokeratin 18 is a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8 and is widely expressed in simple epithelial tissues in adults, where it is found in the cytoplasm, nucleus, and nucleolus. Cytokeratin 18 is associated with the cytoskeleton and mutations in this protein have been associated with liver disease after environmental insult. Cytokeratin 18 can be modified by glycosylation, phosphorylation (Ser34 and Ser53), acetylation (Ser2), and proteolytic cleavage at amino acids 238 and 397 by caspase family members. The cytokeratin 18 protein interacts with a number of proteins including caspase 3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, and usherin, among others.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human breast carcinoma cell line PMC-42
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified
ELISA, IHC-P, IP - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 4.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The DA-7 monoclonal antibody recognizes human cytokeratin 18 and is useful for Western blotting. Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistry of paraffin-embedded sections, and ELISA.
This clone and BioLegend clone LDK18 displayed similar affinities when tested for western blot applications -
Application References
(PubMed link indicates BioLegend citation) -
- Taylor-Papadimitriou J, et al. 1989. J. Cell Sci. 94:403.
- Lauerova L, et al. 1988. Hybridoma 7:495.
- Product Citations
-
- RRID
-
AB_439770 (BioLegend Cat. No. 628401)
AB_439771 (BioLegend Cat. No. 628402)
Antigen Details
- Structure
- Type I intermediate filament protein, contains three coiled-coil domains, approximately 48 kD. Heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8.
- Distribution
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Widely expressed in simple epithelial tissues in adults. Can be found in the cytoplasm, nucleus, and nucleolus
- Function
- Intermediate filament protein involved with the cytoskeleton. Mutations in cytokeratin 18 may predispose to liver disease upon exposure to some viruses or hepatoxins
- Interaction
- Interacts with a number of proteins including caspase3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, usherin
- Modification
- Phosphorylation (Ser34, Ser53), glycosylation, acetylation (Ser2). Can also be cleaved at amino acid 238 and 397 by caspase family members
- Cell Type
- Epithelial cells
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Antigen References
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1. Ku N, et al. 2003. Proc. Natl. Acad. Sci. 100:6063.
2. Kulesh DA and Oshima RG. 1989. Genomics 4:339.
3. Steinart PM and Roop DR. 1988. Ann. Rev. Biochem. 57:593. - Gene ID
- 3875 View all products for this Gene ID
- UniProt
- View information about Cytokeratin 18 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Cytokeratin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Cytokeratin 18 | DA-7 | WB,ICC,ELISA,IHC-P,IP |
Alexa Fluor® 647 anti-Cytokeratin 18 | DA-7 | ICC |
Alexa Fluor® 594 anti-Cytokeratin 18 | DA-7 | ICC,IHC-P |
Alexa Fluor® 488 anti-Cytokeratin 18 | DA-7 | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Cytokeratin 18
Whole cell lysates (15 µg protein) from A549, A431, HeLa, HE... MCF-7 cell extract was resolved by electrophoresis, transfer... MCF-7 cells were stained with anti-Cytokeratin 18 (clone DA-... -
Alexa Fluor® 647 anti-Cytokeratin 18
MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for 10... -
Alexa Fluor® 594 anti-Cytokeratin 18
MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for 10... Human paraffin-embedded colon tissue was prepared with stand... -
Alexa Fluor® 488 anti-Cytokeratin 18
MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for te...
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