- Clone
- W18139A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- EML4, Echinoderm microtubule-associated protein-like 4
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W18139A_PURE_EML4_Antibody_1_091019.png W18139A_PURE_EML4_Antibody_1_091019.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W18139A_PURE_EML4_Antibody_1_092319.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-EML4 Antibody, clone W18139A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Cell lines were loaded in order of increasing predicted EML4 expression (source: Human Protein Atlas). -
HepG2 cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with ice-cold Methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:100 dilution (5.0 µg/mL) of either Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400602, panel A) or Purified anti-EML4 Antibody, clone W18139A (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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670801 | 25 µg | $101 | ||||
670802 | 100 µg | $253 |
Echinoderm microtubule-associated protein-like 4 or EML4 plays a central role in the modification of microtubule structural dynamics and morphology. During mitosis, EML4 expression is upregulated and is associated with spindle formation. In lung cancer cells, a translocation mutation is associated with the EML4 gene, where parts of the gene fuses with the anaplastic lymphoma receptor tyrosine kinase (ALK) gene and generates an EML4-ALK fusion protein.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human EML4 protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1.0 µg/mL. For immunocytochemistry, a concentration of 1.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone has not been tested for IP.
- RRID
-
AB_2814524 (BioLegend Cat. No. 670801)
AB_2814525 (BioLegend Cat. No. 670802)
Antigen Details
- Structure
- EML4 is a 981 amino acid protein with a predicted molecular weight of 109 kD
- Distribution
-
Cytoskeleton/Ubiquitously expressed
- Function
- Microtubule formation
- Antigen References
-
- Yoshida A, et al. 2011. J Thorac Oncol. 10:1677-86.
- Soda M, et al. 2007. Nature. 448:561.
- Pollmann M, et al. 2006. Exp Cell Res. (17):3241-51.
- Gene ID
- 27436 View all products for this Gene ID
- UniProt
- View information about EML4 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All EML4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-EML4 | W18139A | WB,ICC |
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