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Purified anti-Gemin 3 Antibody

Pricing & Availability
Clone
12H12 (See other available formats)
Regulatory Status
RUO
Other Names
Gemin3
Isotype
Mouse IgG1, κ
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Product Citations
publications
12H12_PURE_Gemin3_Antibody_051019
Western blot of purified anti-Gemin 3 antibody (clone 12H12). Lane 1: Molecular weight marker; Lane 2: 20µg of HeLa cell lysate; Lane 3: 10µg of HeLa cell lysate; Lane 4: 5µg of HeLa cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 12H12_PURE_Gemin3_Antibody_051019
    Western blot of purified anti-Gemin 3 antibody (clone 12H12). Lane 1: Molecular weight marker; Lane 2: 20µg of HeLa cell lysate; Lane 3: 10µg of HeLa cell lysate; Lane 4: 5µg of HeLa cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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865501 25 µg $106
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865502 100 µg $276
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Description

Gemin 3, also known as DEAD-box helicase 20 (DDX20), is a putative RNA helicase that contains a conserved ASP-GLU-ALA-ASP (DEAD) motif. Gemin 3 is involved in a variety of cellular processes including initiation of protein translation, ribosome assembly, and alteration of RNA secondary structure. Gemin 3 is one of the core components of the survival of motor neurons (SMN) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. Gemin 3 may also be involved in cancer as it acts as a tumor suppressor in hepatocellular carcinoma, and as a tumor promoter in breast cancer and prostate cancer2.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810775 (BioLegend Cat. No. 865501)
AB_2810776 (BioLegend Cat. No. 865502)

Antigen Details

Structure
Gemin 3 is an 824 amino acid protein with an apparent molecular mass of ~ 92 kD.
Distribution

Tissue Distribution: Testis and ovary
Cellular Distribution: Cytosol, nucleus, and cytoskeleton

Function
Gemin 3 is a component of SMN macromolecule protein complex.
Interaction
SMN, GEMIN4
Cell Type
Astrocytes, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Cancer Biomarkers, Neurodegeneration, Neuroscience, Synaptic Biology, Transcription Factors
Antigen References
  1. Seng CO, et al. 2015. Hum. Mol. Genet. 24(8):2138.
  2. Chen W, et al. 2016. Int. J. Mol. Med. 37(6):1551.
Gene ID
11218 View all products for this Gene ID
UniProt
View information about Gemin 3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/10/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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