Purified anti-HIF1-β Antibody

Pricing & Availability
Clone
W15091A (See other available formats)
Regulatory Status
RUO
Other Names
Aryl hydrocarbon receptor nuclear translocator (ARNT), Class E basic helix-loop-helix protein 2 (bHLHe2), Hypoxia-inducible factor 1-beta
Isotype
Mouse IgG1, κ
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Product Citations
publications
W15091A_PURE_HIF1-beta_Antibody_1_102218
Total cell lysates (15 µg total protein) from THP-1, SH-SY5Y, MCF-7, HeLa, Molt-4, HepG2, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-HIF1-β Antibody, clone W15091A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W15091A_PURE_HIF1-beta_Antibody_1_102218
    Total cell lysates (15 µg total protein) from THP-1, SH-SY5Y, MCF-7, HeLa, Molt-4, HepG2, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-HIF1-β Antibody, clone W15091A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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689602 100 µg $253
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Description

Hypoxia-inducible factor 1 beta (HIF1-β), also known as Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT), functions mainly as a transcription factor. This protein contains a basic helix-loop-helix domain, two PAS (PER-ARNT-SIM) domains and one PAC (PAS-associated c-terminal) domain. HIF1-β binds to the ligand-bound aryl hydrocarbon (Ah) receptor and helps the complex move from cytosol to the nucleus, where it promotes the expression of xenobiotic metabolism-related genes. HIF1-β is also involved in cellular senescence and HIF-1 transcription factor network. It forms a heterodimer with HIF1-α or EPAS1/HIF2A and transcriptionally regulates the response to hypoxia. There are multiple transcript variants of HIF1-β generated by alternative splicing.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human HIF1-β recombinant protein (517-691 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W15091A does not cross-react with mouse.

RRID
AB_2629788 (BioLegend Cat. No. 689602)

Antigen Details

Structure
789 amino acids with a predicted molecular weight of 86.6 kD. Contains a bHLH (basic helix-loop-helix) domain, a PAC (PAS-associated C-terminal) domain, and two PAS (PER-ARNT-SIM) domains.
Distribution

Nucleus

Function
HIF1-β interacts with ligand-bound aryl hydrocarbon receptor (AhR) and acts as a transcriptional co-activator for the genes involved in xenobiotic metabolism.
Interaction
Forms a heterodimer with AHR, AHRR, HIF1A, EPAS1/HIF2, and other bHLH proteins. Interacts with TACC3 and NOCA7.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Furue M, et al. 2014. Arch Dermatol. Res. 306:769.
2. Vorrink SU, et al. 2014. Chem. Biol. Interact. 218:82.
3. Mandl M, et al. 2014. Mol. Med. 20:215.
4. Gu C, et al. 2014. Leuk. Res. 37:1750.
5. Wolff M, et al. 2013. Cell Physiol Biochem. 32:849.
6. Nakajima K, et al. 2013. Nat. Commun. 4:2112.

Gene ID
405 View all products for this Gene ID
UniProt
View information about HIF1-beta on UniProt.org
Go To Top Version: 2    Revision Date: 10/22/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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